Fig 1.
Compounds explored in this study.
(A) Formamidine and (B) formamidine urea compounds with the code number for the most cytotoxic in red and the least cytotoxic in blue.
Table 1.
Formamidine and formamidine urea compounds explored in this study.
Table 2.
LC50 values for the amidine derivatives extracts.
Fig 2.
Optical microscopy images of Vero (left column) and COS-7 (right column) cells exposed to 24 h-extracts.
(A) Control cells, (B) compound 2 (1.25 mg/mL, 3.7 mM), (C) compound 5 (1.25 mg/mL, 2.9 mM), and (D) compound 10 (4.25 mg/mL, 13.3 mM), each for 24 h. Scale bar = 200 µm.
Fig 3.
SEM images of Vero cells exposed to 24 h-extracts.
(A) Untreated (control) cells, (B) compound 5 at 1.25 mg/mL and (C) compound 10 at 4.25 mg/mL for 24 h. In (A) the arrows show healthy cell-substrate and cell-cell interactions; in contrast, in (B) and (C), * indicates cell detachment, while ** the loss of the interactions between the cell and other cells or the substrate.
Fig 4.
SEM images of COS-7 cells exposed to 24 h-extracts.
(A) Untreated (control) cells, (B) compound 5 at 1.25 mg/mL and (C) compound 10 at 4.25 mg/mL for 24 h. In (A) the arrows show healthy cell-substrate and cell-cell interactions; in contrast, in (B) and (C), * indicates cell detachment/rounding, while ** the loss of the interactions between the cell and other cells or the substrate.
Fig 5.
Values measured for the 24 h DMEM extract (A) and the 7-day DMEM extracts (B) in contact with four different cell lines for 24 h.