Table 1.
qRT-PCR primer information.
Fig 1.
Dynamic changes in embryo development rate of Aconitum kusnezoffii seeds during stratification.
Fig 2.
Dynamic changes in the contents of six endogenous hormones during seed dormancy release in Aconitum kusnezoffii: (a) Abscisic acid (ABA); (b) Gibberellin A₃ (GA₃); (c)Indole-3-acetic acid (IAA); (d) Methyl jasmonate (MeJA); (e) Brassinosteroid (BL); (f) Zeatin riboside (ZR; reported as the sum of trans- and cis-isomers, tZR + cZR).
Each value represents the mean ± standard deviation (n = 3). Different lowercase letters indicate statistically significant differences among stages (p < 0.05).
Fig 3.
Dynamic changes in the ratios of key endogenous hormones to ABA during seed dormancy release in Aconitum kusnezoffii: (a) GA₃/ABA ratio; (b) IAA/ABA ratio; (c) ZR/ABA ratio.
Each value represents the mean ± standard deviation (n = 3). Different lowercase letters indicate statistically significant differences among stages (p < 0.05).
Table 2.
Statistics of sequencing data of Aconitum kusnezoffii.
Fig 4.
Summary of differentially expressed genes (DEGs) identified during seed dormancy release in Aconitum kusnezoffii: (a) Number of upregulated and downregulated DEGs in each comparison group (T1_vs_T2, T1_vs_T3, T2_vs_T3); (b) Venn diagram showing the overlap of DEGs among the three comparison groups.
Fig 5.
KEGG enrichment analysis of differentially expressed genes (DEGs) during seed do.
Fig 6.
KEGG pathway enrichment analysis of differentially expressed genes (DEGs) from five significant expression profiles identified by STEM clustering in Aconitum kusnezoffii seeds during dormancy release.
Fig 7.
Expression patterns of key genes involved in the ABA biosynthesis, metabolism, and signal transduction pathways during seed dormancy release in Aconitum kusnezoffii.
Fig 8.
Expression profiles of key genes involved in GA biosynthesis, deactivation, and signal transduction during seed dormancy release in Aconitum kusnezoffii.
Fig 9.
Expression dynamics of key genes involved in auxin (IAA) biosynthesis and signal transduction during seed dormancy release in Aconitum kusnezoffii.
Fig 10.
Alidation of RNA-seq data by qRT-PCR for 14 selected genes involved in hormone signaling and metabolism in Aconitum kusnezoffii.