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Table 1.

Oxygen diffusion and consumption parameters.

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Fig 1.

Schematic of the simulation environment including microchannel distribution.

A 2D tissued grid with randomly positioned cancer stem cell (CSC) and oxygen microchannels shown as white dots. The model simulates CSC behavior under spatial and oxygen constraints.

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Table 2.

Sensitivity analysis of a00.

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Table 3.

Sensitivity analysis of a01.

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Table 4.

Sensitivity analysis of migration.

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Fig 2.

Simulated tumor growth initiated by a single cancer stem cell after 20 days.

Blue represents CSCs; red indicates two phenotypic types of non-stem cells. The tumor exhibits hierarchical expansion and local heterogeneity.

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Fig 3.

Time course of CSC and non-CSC population expansion.

The number of CSCs and non-stem cancer cells over simulation time, reflecting asymmetric and symmetric division dynamics with emergence of hierarchical tumor structure.

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Fig 4.

Histogram of cell migration frequency during tumor development.

Most cells exhibit minimal migration, with a few undergoing multiple migrations- matching a power law distribution pattern.

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Fig 5.

Reference power-law distribution for comparison.

A generic power-law function is used as a conceptual benchmark for interpreting cell migration distribution in the simulation.

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Fig 6.

Spatial distribution of cell location by migration frequency.

Cells with higher migration counts tend to spread further from the origin. CSCs are shown as circles, non-CSCs as triangles.

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Fig 7.

Cross-sectional oxygen concentration map in simulated tissue.

Oxygen gradients reflect diffusion from microchannels and local consumption. Hypoxic zones emerge where tumor density increases.

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Fig 8.

Oxygen concentration fluctuates in cancerous vs. non-cancerous regions.

Pixel-wise oxygen levels plotted to demonstrate depletion caused by tumor metabolic activity and spatial constraints.

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Fig 9.

Experimental tumor growth data from Norton er al.

[10]. Shows CSC-driven tumor progression in vivo used for model validation.

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Table 5.

Non-divergence and stability analysis of model.

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Table 6.

Key similarities and differences.

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Fig 10.

Comparison between experimental and simulated tumor area growth.

Model prediction closely matches biological data in both shape and saturation behavior.

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Table 7.

Numerical validation between experimental and simulation data.

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Table 8.

Quantitative results of Q-learning therapy.

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Table 9.

Comparison with previous Models.

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