Fig 1.
Predicted 3D structures and inferred functions of SBiP1-interacting proteins.
Three-dimensional models of candidate SBiP1-interacting proteins identified by yeast two-hybrid screening. Predictions were generated using AlphaFold2 (ColabFold implementation) and assessed by per-residue confidence scores (pLDDT). Structural similarity searches were performed using Foldseek to infer putative functions. Models include HSP70 (C1), POX18 (C2/5/10), an unannotated globular α-helical protein (C3), TARBP1 (C4/11) with structural similarity to NOP58, MAP2B (C6), GTPBP1 (C7), WRAP73 (C8), and EFL (C9).
Table 1.
Summary of SBiP1-interacting proteins identified by Yeast Two-Hybrid screening.
Fig 2.
Basal expression of SBiP1 during a time course under standard culture conditions.
Relative transcript levels of SBiP1 were measured at five time points (days 3, 6, 9, 12, 15) under standard culture conditions. Values were normalized to SmicEF1-α and calculated using the 2 ⁻ ΔΔCt method with day 3 as calibrator. Bars represent mean ± SE (n = 9 per time point). Different letters above bars indicate statistically significant differences (one-way ANOVA followed by Tukey’s HSD, α = 0.05).
Fig 3.
Expression of SBiP1 under light and dark conditions.
Transcript levels of SBiP1 at days 3, 12, and 18 in cultures maintained under light or dark conditions. Values were normalized to SmicEF1-α and expressed relative to day 3 (light). Bars represent mean ± SE (n = 9, from 3 biological replicates × 3 technical replicates treated as independent values). Different letters indicate significant differences between groups (two-way ANOVA, Tukey’s HSD, α = 0.05).
Fig 4.
Basal expression profile of POX18 during a time course under standard conditions.
Relative transcript levels of POX18 across six time points, normalized to SmicEF1-α and expressed relative to day 3. Bars represent mean ± SE (n = 9 per time point). Different letters above bars indicate significant differences (one-way ANOVA followed by Tukey’s HSD, α = 0.05).
Fig 5.
Basal expression profile of HSP70 during a time course under standard conditions.
Relative transcript levels of HSP70 across six time points, normalized to SmicEF1-α and expressed relative to day 3. Bars represent mean ± SE (n = 9 per time point). Different letters above bars indicate significant differences (one-way ANOVA followed by Tukey’s HSD, α = 0.05).
Fig 6.
Effect of cycloheximide on the expression of SBiP1, HSP70, and POX18.
Transcript levels of SBiP1, HSP70, and POX18 at day 12 in control and CHX-treated cultures. Expression values were normalized to SmicEF1-α and expressed relative to untreated controls. Bars represent mean ± SE (n = 3 biological replicates, with technical triplicates averaged within each biological). The asterisk indicates significant difference between treated and control groups (p < 0.05, Welch’s t-test).