Table 1.
Treatment groups and survival rates in the extended single-dose toxicity assay for CTX/PMB.
Fig 1.
Mice body weight (A.1) was monitored over 14 days following a single dose of CTX/PMB at three different CTX concentrations (low, mid, and high) or vehicle (control).
The area under the weight curves was calculated for each group (A.2). Food consumption was recorded daily for all groups (B.1), and corresponding areas under the food consumption curves were obtained (B.2). Water consumption was monitored over the same period (C.1), and the area under the curves was calculated for each group (C.2). Area under the curve (AUC) values were analyzed for statistical significance using one-way ANOVA, with p < 0.05 considered significant.
Table 2.
Mice organ weights 14 days after receiving a single dose of CTX/PMB or vehicle.
Fig 2.
The biochemical markers in mice treated with CTX/PMB at three different CTX doses (low, medium, and high) and the control group (vehicle only) were quantified.
The parameters included A) creatinine, B) urea, C) alanine aminotransferase (ALT), D) alkaline phosphatase, E) aspartate aminotransferase (AST), F) total bilirubin, G) direct bilirubin, and H) indirect bilirubin. The dashed lines indicate the upper reference limits for each parameter. Statistical analysis was performed via one-way ANOVA; no significant differences were found among the groups.
Fig 3.
A) Schematic representation of the PC-R K. pneumoniae K18 strain infection model in mice used to evaluate the antimicrobial efficacy of the CTX/PMB combination against a resistant strain.
B) Survival curves of mice treated with PMB, CTX, or the CTX/PMB combination following PC-R K. pneumoniae K18 infection. C) Bacterial load (log10 CFU/mL) across treatment groups. A lower detection limit of 1.69 log10 CFU/mL was established. Naïve (uninfected) and mock-treated (infected but untreated) control groups were included. Statistical analysis was performed via one-way ANOVA; the differences among groups were considered to be statistically significant at p < 0.05.