Table 1.
Mean (±SE) comparisons between genotypes of SCD SNP g.23881050T > C for docosahexaenoic acid (DHA), docosapentaenoic acid (DPA) and intramuscular fat (IMF) content in Tattykeel Australian White (TAW) lambs.
Table 2.
Primer parameters of the SCD polymerase chain reaction assay.
Table 3.
SCD genotypes and allele frequencies in Tattykeel Australian White (TAW) rams, ewes, young rams, poll Dorset, Martin More and Angus bull.
Fig 1.
The amplification curves illustrating the genotypic profiles of homozygotes and heterozygote for both alleles of the SCD gene in Tattykeel Australian White (TAW), Poll Dorset and Martin More sheep, and Angus bull populations, showing precision and consistency in genetic analysis of 118 samples (A, n = 68; B, n = 50).
Fig 2.
Allelic discrimination plot for SNP g.23881050T > C in the SCD gene, generated using TaqMan genotyping assays on 118 samples from Tattykeel Australian White (TAW), Poll Dorset, Martin More, and Angus bull breeds (A, n = 68; B, n = 50).
The blue dots indicate homozygous recessive genotype, green dots represent the heterozygous genotypes, black X corresponds to undetermined Angus bull samples, and black squares denote no-template controls.
Table 4.
Major and minor allele frequencies of SCD gene SNP in Tattykeel Australian White (TAW), Poll Dorset, Martin More and Angus bull.
Table 5.
TaqMan SNP assay cost per sample.