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Fig 1.

Canopy temperature (CT) value under Control and combined stress (HD) condition.

Under control conditions, the colors blue, red, and green indicate CT values at anthesis, 10 days after anthesis (DAA), and 20 DAA, respectively. In contrast, under the HD condition, these colors correspond to CT values at anthesis, 5 DAA, and 10 DAA, respectively.

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Fig 1 Expand

Fig 2.

Soil Plant Analysis Development (SPAD) value under Control and HD condition.

Blue, red and green colors representing the SPAD values at Anthesis, 10 DAA (Days after anthesis) and 20 DAA, respectively under control condition. Under HD condition these colors representing the SPAD value at Anthesis, 5 DAA and 10 DAA, respectively.

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Fig 2 Expand

Fig 3.

Leaf senescence rate (LSR) and Thousand grain weight (TGW) under Control and combined stress (HD) condition.

Blue and red color representing the control and HD condition, respectively.

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Fig 4.

Chlorophyll a, b, chl a/b, total chlorophyll and total carotenoids content under Control and HD condition.

Under control conditions, the colors blue, red, and green indicate at anthesis, 10 days after anthesis (DAA), and 20 DAA, respectively. In contrast, under the HD condition, these colors representing at anthesis, 5 DAA, and 10 DAA, respectively.

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Fig 4 Expand

Fig 5.

Stem reserve mobilization efficiency (SRE) percentage under Control and HD condition.

Blue and red color indicates the control and HD condition, respectively.

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Table 1.

The role of the identified putative candidate genes in the regulation of staygreen and stem reserve mobilization traits.

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Table 1 Expand

Fig 6.

A. Heatmap of relative gene expression for canopy temperature.

TaPD (TraesCS3B02G563100), TaEIF-3 (TraesCS3D02G506600), TaPP2C (TraesCS2D02G214200), TaFBP (TraesCS7A02G017800), TaCSL (TraesCS5A02G036300); after gene name numbers 1, 2, and 3 represents the expression levels during stage-1 (50% Anthesis), Stage-2 (5DAA), Stage-3 (10 DAA), respectively. B. Heatmap of relative gene expression for Soil and plant analysis development (SPAD) value. TaE3 ubiquitin (TraesCS3D02G018100), TaPPR (TraesCS3D02G018200), TaCKX11 (TraesCS2A02G378300); after gene name numbers 1, 2, and 3 represents the expression levels during stage-1 (Anthesis), stage-2 (5DAA), stage-3 (10 DAA), respectively. C. Heatmap of relative gene expression for Normalized difference vegetation index (NDVI). TaMYB (TraesCS5D02G385000), TaCAAT-like (TraesCS5D02G385125); after gene name numbers 1, 2, and 3 represents the expression levels during stage-1 (Anthesis), stage-2 (5DAA), stage-3 (10 DAA), respectively. D. Heatmap of relative gene expression for Stem Reserve Mobilisation Efficiency (SRE). TaWAR (TraesCS6B02G458200), TaSK2 (TraesCS2D02G053300), TaABC (TraesCS2A02G056800), TaUGT (TraesCS2A02G056100) expression levels in peduncle.

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Fig 6 Expand