Table 1.
The Number of SL Biosynthetic Genes in Different Species.
Fig 1.
Phylogenetic tree of SL biosynthetic proteins from nine Solanaceous species.
Proteins are color-coded by gene family and labeled by species, as indicated in the legend.
Fig 2.
Phylogenetic tree of SL biosynthetic genes from nine Solanaceous species.
The gene structures of SL biosynthesis-related genes—including D27, CCD7, CCD8, LBO, CYP711A, CYP712G, CYP722C, CYP728B, and CYP706C—were visualized based on genomic annotations. Each gene model is depicted with colored boxes representing exons, thin lines for introns, and arrows indicating transcriptional direction.
Fig 3.
Classification and distribution of cis-regulatory elements in SL biosynthetic gene promoters across different species.
Elements were grouped into four functional categories: stress responsiveness, hormone responsiveness, developmental regulation and light responsiveness. The outermost circular axis lists gene IDs from different species, while rows represent individual cis-elements. Color intensity indicates the number of element occurrences, as shown in the legend.
Table 2.
Selection pressure analysis of homologous gene pairs.
Fig 4.
Predicted 3D protein structures of SL biosynthetic genes in tomato and pepper.
This figure presents the AlphaFold-predicted 3D structures of proteins encoded by SL biosynthesis-related genes from Solanum lycopersicum (Solyc) and Capsicum annuum (Capana). Panels A–H display tomato proteins, and panels I–Q show corresponding proteins from pepper.
Fig 5.
Relative expression levels of SL biosynthetic genes under normal (N) and Pi-deficient (L) conditions in tomato (A) and pepper (B).
Gene expression levels were quantified by qRT-PCR and calculated using the 2^-ΔΔCT method. ** significant differences between treatments at p < 0.01; ns: no significant difference. Data represent the mean ± SD for three biological replicates (n = 3).