Fig 1.
Taxa bar plot of intestinal microbiota from healthy and diseased shrimp at the genus level.
The relative abundance of each intestinal microbiota is shown. Each bar represents the data of a pooled intestinal sample in each group (n = 5).
Fig 2.
Heat map of intestinal microbiota from healthy and diseased shrimp at the genus level.
Each column represents the data of a pooled intestinal sample in each group (n = 5). Color indicated on the map represents the relative abundance of each intestinal microbiota: Red coloration represents higher relative abundance, while blue coloration represents lower relative abundance. Clustering analysis is shown as tree on the left side of the map.
Fig 3.
Venn diagram based on ASVs of intestinal microbiota from healthy and diseased shrimp.
Number on blue and orange coloration circles represent unique ASVs of intestinal microbiota in healthy and diseased shrimp, respectively, while number on the overlap circle represents shared ASVs of intestinal microbiota in healthy and diseased shrimp. Data were generated based on 5 pooled intestinal samples from each group (n = 5).
Fig 4.
α-diversity indices of intestinal microbiota from healthy and diseased shrimp.
(A) Observed features, (B) Dominance, (C) Shannon, and (D) Simpson. Data were generated based on 5 pooled intestinal samples from each group (n = 5).
Fig 5.
β-diversity of intestinal microbiota from healthy and diseased shrimp.
PCoA of (A) un-weighted and (B) weighted UniFrac distance with PERMANOVA P-value. Boxplot of ANOSIM based on (C) un-weighted and (D) weighted UniFrac distance with P- and R-value. Data were generated based on 5 pooled intestinal samples from each group (n = 5).
Fig 6.
Functional feature prediction of intestinal microbiota from healthy (blue) and diseased (orange) shrimp.
Fisher’s exact-test was employed to find significant differences of data between groups. P-value of the data was corrected using Newcombe-Wilson CI method and Benjamini-Hochberg FDR. Only the pathways with either significantly enhanced or suppressed by the occurrence of pale shrimp disease are shown. Data were generated based on 5 pooled intestinal samples from each group (n = 5).