Table 1.
The genes and primer sequences used for the amplification of the samples analyzed in the present study, in the 5’ → 3’ direction.
Table 2.
Molecular data and BOLD Systems accession numbers for the analyzed specimens. Specimens marked with an * were not sequenced by Sanger.
Fig 1.
Electrophoretic gel showing the quality of the genomic DNA extracted from the samples (cytogenetic suspensions) processed in the present study.
The sample numbers correspond to those in Table 2. Electrophoresis was performed under constant voltage (120 V) for 30 minutes, using a 1 Kb DNA ladder as molecular size marker.
Fig 2.
Electrophoretic gel showing the PCR products generated by the primers for the COI, 5S rDNA, 18S rDNA, and RAG2 genes, applied to DNA extracted from samples of cytogenetic suspension fixed in Carnoy’s solution.
The sample numbers correspond to those in Table 2. NC = Negative Control. Electrophoresis was performed at 90 V for 40 minutes, using a 100 bp DNA ladder as molecular size marker.