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Fig 1.

Geographical distribution of the eight divisions of Bangladesh where tick-borne bovine oriental theileriosis was investigated.

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Table 1.

Prevalence of T. orientalis in cattle across all divisions of Bangladesh.

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Table 2.

BLAST (Basic Local Alignment Search Tool) analysis of Theileria orientalis MPSP gene sequences from cattle in Bangladesh showing query accession numbers, genotypes, closest GenBank matches, identity percentages, and countries of origin.

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Table 3.

Intra-sequences (query) similarity matrix.

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Fig 2.

Maximum-likelihood phylogenetic tree of Theileria orientalis MPSP gene sequences with Babesia bigemina (M85187.1) as the outgroup.

Sequences from this study are marked with triangles (▲ black = Genotype 7; ▲ red = Genotype 5). Labels include accession numbers (in parentheses), host, country and the numbers along with branches indicate the bootstrap values.

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Table 4.

Haplotype-nucleotide diversity and neutrality tests of MPSP gene sequences of T. orientalis.

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Table 5.

Gene flow and genetic differentiation indices between two genotypes of T. orientalis estimated by MPSP gene sequence.

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Table 6.

Estimation of codon-based evolutionary divergence between MPSP sequences.

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Table 7.

List of Theileria orientalis haplotypes identified based on MPSP gene sequences in cattle from eight divisions of Bangladesh. The table includes haplotype number, number of sequences, genotypes, geographic location and their corresponding GenBank accession numbers.

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Fig 3.

Median Joining Network indicates haplotype relationships and genetic diversity of T. orientalis based on MPSP gene sequences.

The figure depicts a constructed Median Joining Network based on haplotype analysis of the MPSP gene sequences. Each circle represents a haplotype, with the size proportional to the number of samples sharing that haplotype. The colors indicate different haplotypes, highlighting genetic differentiation among groups. The hatch marks on the connecting lines between haplotypes represent the mutational steps separating them.

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