Fig 1.
Flow chart summarizing the study design and laboratory analyses.
Fig 2.
Example of the result obtained on the MEM-G6PD membrane of the Hybribio G6PDd test.
Results were interpreted based on colored dots appearing on specific probes of the MEM-G6PD membrane: mutant (M) probes for homozygous/hemizygous samples, both M and normal (N) probes for heterozygous samples, and only N probes for normal/undetected samples. A biotin control spot is shown in the bottom box (far right). The exact positions of these dots, and the mutations they represent, are provided in the kit protocol.
Table 1.
Frequency of cases and range of G6PD activities measured by the OSMMR2000-D test (n = 258).
Table 2.
G6PD mutation variants detected by the Hybribio G6PDd test among the Proto Malay Orang Asli population (n = 73).
Fig 3.
Tree diagram showing the results of the Hybribio G6PDd test.
A total of 38 samples exhibited mutation variants: G6PD Viangchan (c.871G > A) (14 cases), G6PD Coimbra (c.592C > T) (12 cases), G6PD Union (c.1360C > T) (10 cases), and G6PD Kaiping (c.1388G > A) (2 cases).
Table 3.
Allelic frequencies of G6PD mutations among the Proto Malay Orang Asli deficient and intermediate samples (<80% of AMM) (n = 34).
Table 4.
Allelic frequencies of G6PD mutations among the Proto Malay Orang Asli normal samples (>80% of AMM) (n = 6).
Table 5.
G6PD mutation variants among the three main groups of the Malaysian Orang Asli population.