Table 1.
Morphological and biochemical characteristics of bacterial isolates.
Fig 1.
Scanning electron microscopy (SEM) images of L. boronitolerans MSR1 showing surface morphology at different magnifications.
(a) 10,000x, (b) 15,000x, (c) 25,000x, and (d) 40,000x.
Fig 2.
Growth of L. boronitolerans MSR1 in different pH (5-8).
(a) Growth patterns observed at pH 5 and 6; (b) Growth patterns observed at pH 7 and 8, highlighting variations in developmental stages across conditions.
Fig 3.
Phylogenetic tree analysis for species identification.
(a) Whole genome-based phylogeny; (b) 16s sequence-based phylogeny.
Table 2.
Genomic properties of isolated strain.
Fig 4.
A circular genome map of L. boronitolerans MSR1.
This map displays various genomic features in a layered circular format. The concentric circles represent different aspects of the genome, starting from the outermost ring, which indicates annotated genes (CDS) and functional elements, while the inner rings show the GC content, GC skew, and repeat regions. Each color denotes a specific genomic feature: GC content is shown in purple, while CDs, tRNA, and rRNA are marked in distinct colors. The annotations around the outer ring point to specific genes and proteins encoded by the genome, including important enzymes like acetyltransferase, hypothetical proteins, and reductases, highlighting functional diversity.
Fig 5.
(a) ANI Matrix. Nucleotide identity comparison between the strains of Lysinibacillus boronitolerans. The ligand on the top left indicates a similarity between blue (low) and orange (high). (b) Circular genome comparison of Lysinibacillus boronitolerans strains. The red-marked region is the unique region of L. boronitolerans MSR1.
Table 3.
Variants in L. boronitolerans MSR1.
Table 4.
Major BGC and their position in the genome of 7NBS.
Fig 6.
Each region presents a separate position for BGCs in the genome.
Table 5.
Number of horizontally transferred genes (HGT) detection.
Fig 7.
Horizontally transferred genes.
Plot indicating the distributions of “close” and “distal” scores revealed by similarity searches. The scatter plot was created with HGTector. Potentially transferred genes (n = 102) are indicated in yellow.
Table 6.
Antibiotic susceptibility of L. boronitolerans MSR1.
Table 7.
Major AMR genes.
Table 8.
AMR genes from HGT event.
Fig 8.
AMR gene’s position in the genome of L. boronitolerans MSR1.
Red ones are strict hits, and blue ones are loose hits from CARD database.
Table 9.
Predicted plasmid from L. boronitolerans MSR1 genome.
Table 10.
Pathogenicity prediction of L. boronitolerans MSR1.
Table 11.
Percentage of core, shell and cloud genes L boronitolerans strains.
Table 12.
Number of different genes in each L boronitolerans strain.
Fig 9.
(a) Roary prediction of the overall core, shell and cloud gene analysis. (b) Number of core, accessory and unique genes present in individual strains.
Fig 10.
Pan genome analysis of L. boronitolerans.
(a) Pan-plot of L. boronitolerans core and pan-genome. (b) Gene absence/presence matrix of L. boronitolerans genomes.
Fig 11.
(a) Major KEGG pathways of core, accessory, and unique genes. (b) Major COG distribution of core, accessory, and unique genes.
Fig 12.
Phylogenetic relation among L. boronitolerans strains based on pan-genome analysis.
(a) Core Phylogenetic tree of L. boronitolerans strains based on core gene; (b) Pan phylogenic tree based on pan-genome of L. boronitolerans. The digits present the branch lengths.