Fig 1.
Preparation Steps for LN@AgNPs (change of color from transparent to brown showed that nanoconjugation occurred).
Fig 2.
Ultraviolet-visible spectroscopy: SPR bands spectrum of LN@AgNPs.
Fig 3.
Particle size determination of synthesized nanoparticles.
(a) size distribution by intensity (b) size distribution histogram based on number data.
Fig 4.
Zeta potential measurement: Analysis of zeta potential of synthesized silver nanoconjugates of Linalool.
Fig 5.
FTIR spectrum of LN@AgNPs: Graph with wavelength (cm-1) on the x-axis and transmittance percentage on the y-axis.
Fig 6.
SEM image of LN@AgNPs: Magnified image of selected area (left) with number of particles with respective area (right).
Fig 7.
EDAX spectrum of LN@AgNPs.
Fig 8.
Interaction diagram of ligands.
(a-b LN@AgNPs, c. Linalool) to binding site of Glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) of E.coli (6IO4).
Fig 9.
Interaction diagram of ligands.
(a. LN@AgNPs, b. Linalool) to binding site of FtsZ B. of Bacillus subtilis (2VAM) and ligands (c. LN@AgNPs, d. Linalool) to binding site of type IVb pilin from Salmonella enteric (3FHU).
Table 1.
Molecular interaction Profile of LN@AgNPs and individual linalool with selected targets.
Fig 10.
A microtiter plate showing minimum inhibitory concentration (MIC): Score growth in test wells presence/absence of turbidity (1. Positive control, 2. Negative control, 3.Linalool and 4. LN@AgNPs) against Salmonella enterica, E.coli and Bacillus subtilis.
Table 2.
Determination of MIC (μL/mL) and %Inhibition by microdilution method.
Fig 11.
Antibacterial activity evaluating using Agar-well diffusion method: micro plates for bacterial strains Salmonella enterica, E.coli and Bacillus subtilis with zone of inhibitions labeled with negative control (c), positive control (AZM), Linalool (LN) and LN@AgNPs (Left) and bar-graph showing zone of inhibitions (right).
Fig 12.
Antibacterial activity evaluating using Disc diffusion method: micro plates for bacterial strains; Salmonella enterica, E.coli and Bacillus subtilis with zone of inhibitions labeled with negative control (c), positive control (AZM), Linalool (LN) and LN@AgNPs (Left) and bar-graph showing zone of inhibitions (right).