Table 1.
Patients and control group characteristics.
Fig 1.
Chloride intracellular channels genes expression in the head and neck squamous cell carcinoma tumors and normal tissues at the mRNA and protein levels.
All analyses were measured with the Mann-Whitney U test. A. CLIC1 – p = 0.0388, CLIC2 – p = 0.0493, CLIC3 – p < 0.0001, CLIC5 – p < 0.0001. B. CLIC3 – p < 0.0001. T – tumor tissue, N – normal tissue. C. CLIC1 oral cavity – p = 0.0398, CLIC2 larynx – p = 0.0217, CLIC3 larynx and oral cavity – p < 0.0001, CLIC4 oral cavity – p = 0.0338, CLIC5 oral cavity – p < 0.0001, CLIC6 oral cavity – p = 0.0006. D. CLIC3 oral cavity – p < 0.0001.
Table 2.
Chloride intracellular channel family members expression in oral cancer.
Fig 2.
Chloride intracellular channel genes mRNA expression in the head and neck squamous cell carcinoma tumors and normal tissues, depending on the tumor staging and the histological grade.
All analyses were measured with the Kruskal-Wallis test and Dunn’s multiple comparison test: Tumor staging-CLIC1 – p = 0.0018, T2 vs. T3 – p = 0.0007, T2 vs. T4 – p = 0.0388; Tumor staging-CLIC6 – p = 0.039, T1 vs. T4 – p = 0.0434; Histological grade-CLIC6 – p = 0.0004, G2 vs. G3 – p = 0.0031.
Fig 3.
Chloride intracellular channel blood serum protein expression in oral squamous cell carcinoma patients and the control group.
A. All analyses were measured with the unpaired t-test: CLIC1 – p = 0.0023, CLIC3 – p < 0.0001, CLIC4 – p = 0.0122, CLIC6 – p = 0.0015. B. CLIC1 – Kruskal-Wallis test – p = 0.0121, Dunn’s multiple comparison test: Control vs. T2 – p = 0.0228, Control vs. T4 – p = 0.031), CLIC3 – ANOVA – p < 0.0001, Tukey’s multiple comparison test: Control vs. T2 – p = 0.0022, Control vs. T3 – p = 0.0004, Control vs. T4 – p = 0.0001. CLIC6 – ANOVA – p = 0.0055, Tukey’s multiple comparison test: Control vs. T2 – p = 0.0035, Control vs. T3 – p = 0.033. C. All analyses were measured with ANOVA and Tukey’s multiple comparisons test: CLIC1 – p = 0 < 0001, Control vs. N0 – p < 0.0001, Control vs. N1 – p = 0.0010, Control vs. N2 – p = 0.0365, N0 vs. N3 p = 0.0049; CLIC2 – p = 0.0082, N0 vs. N2 – p = 0.0082, N0 vs. N3 – p = 0.0377; CLIC3 – p = 0.0281, Control vs. N1 – p = 0.0427; CLIC5 – p = 0.0405, Control vs. N3 – p = 0.0337. D. All analyses were measured with ANOVA and Tukey’s multiple comparisons test: CLIC1 – p = 0.0008, Control vs. G1 – p = 0.0006, Control vs. G2 – p = 0.0055; CLIC3 – p < 0.0001, Control vs. G2 – p < 0.0001, Control vs. G3 – p = 0.0034; CLIC6 – p = 0.0046, Control vs. G2 – p = 0.0192, Control vs. G3 – p = 0.0034.
Table 3.
The proposed cut-off levels of the chloride intracellular channels protein blood serum concentration, and the corresponding sensitivity and specificity in diagnosing oral squamous cell carcinoma.
Table 4.
Original studies regarding chloride intracellular channels in head and neck cancer.