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Fig 1.

Map showing: (A) the geographic location of Osijek (base map credit: USGS National Map Viewer, https://apps.nationalmap.gov/viewer/); (B) the exact location of the well SU 233/234 during the excavation (in red circle).

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Fig 2.

The well SU 233/234 during different excavation phases showing the position of the skeletons: (A) SK 2, SK 3 and SK 4; (B) SK 5 and SK 7.

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Fig 3.

(A) Broken maxillary central right incisor; (B) cut to the diaphysis of the left humerus, detail, SK 4.

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Fig 4.

(A) Puncture wound on the anterior side of the manubrium, SK 4; (B) puncture wound on the posterior side of the right ilium, SK 5.

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Fig 5.

The chart showing C/N stable isotope values for the individuals from Mursa (the faunal baseline adapted from Lightfoot et al.).

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Fig 6.

A West-Eurasian PCA projecting from newly generated and previously published ancient individuals from Mursa and Croatian Iron Age onto the modern-day genetic diversity.

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Fig 7.

Modelling of ancient individuals from Mursa using proximal sources in qpAdm.

The pre-Roman local ancestry is represented by Croatia Early Iron Age (Croatia_EIA.AG), the Eastern Mediterranean ancestry by Turkey Iron Age (Turkey_Aegean_Bodrum_Halikarnassos_Ancient1.AG), the Northern European ancestry by Denmark Iron Age (Denmark_IA.SG), and the Eastern European ancestry by Ukraine Early Iron Age (Ukraine_Dnieper_EIA.SG). These sources are chosen from a range of viable models and serve as representative population of the distinct ancestries found in Mursa.

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