Fig 1.
Differential gene expression analysis in OA and OP.
The Volcano diagram of Differential expression genes in (A) GSE169077, (B) GSE55457 and (C) GSE35958; The heatmap of differently expressed genes in (D) GSE169077, (E) GSE55457 and (F) GSE35958 datasets across various samples.
Fig 2.
(A) GO Enrichment analysis results for up-regulated genes and (B) down-regulated genes; (C) GO network analysis results for down-regulated genes and (D) up-regulated genes.
Fig 3.
KEGG pathway enrichment analysis.
(A)The KEGG pathway enrichment analysis results for up-regulated genes and (B) down-regulated genes; (C) The KEGG network analysis results for down-regulated genes and (D) up-regulated genes.
Fig 4.
Venn Diagram of (A) Down-regulated genes and (B) Up-regulated genes; Expression of overlapping genes in datasets (C) GSE169077, (D) GSE55457, and (E) GSE35958; (F) Genes associated with ferroptosis.
Fig 5.
Elevation of ferroptosis levels in BMSCs during OP progression.
(A)The micro-CT results of mouse bone tissue and (B) Bone volume over tissue volume (BV/TV), number of trabeculae (Tb.N), bone trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were determined. (C) H&E staining results of mouse bone tissue; (D) Flow cytometry analysis of BMSCs apoptosis levels; (E) Statistical analysis of (D); Levels of (F) Fe2+, (G) MDA, and (H) GSH in BMSCs; (H) Levels of SLC2A3, TXNIP, ACSL4, GPX4, and FTH1 in BMSCs; (I) Statistical analysis of (H).