Fig 1.
Photographic phagocytosis assay.
RAW 246.7 cells were exposed to A. baumannii with a multiplicity of infection of 1:20. Cells were given 10 µg/mL of either a non-specific IgG isotype control antibody (A), or one of two experimental MAbs, MAb3 (B) and MAb39 (C) in the presence of 10% CD1 mouse serum. Cells were stained and photographed, and phagocytosis was evaluated using Fiji software. Non-parametric statistical analyses were performed via Mann-Whitney U test (D).
Fig 2.
RAW 246.7 cells were exposed to A. baumannii with a multiplicity of infection of 1:20. Cells were given 10 µg/mL of either a non-specific IgG isotype control antibody or our experimental antibody, MAbC8. Following phagocytosis, RAW 246.7 cells were lysed, and A. baumannii within was collected and quantitatively plated to determine CFU/mL lysate. Non-parametric statistical analyses were performed via Mann-Whitney U test. This data previously published [7].