Fig 1.
(A) Variation in gene expression levels between the samples subjected to normal diet (ND) or high-fat diet (HFD). (B) Distribution of genes based on log fold change and log p-value.
Fig 2.
Top 10 significant functional annotations of up-regulated DEGs.
(A) Biological processes, (B) cellular components, and (C) molecular functions, ranked by adjusted p-values. Functional annotations are ordered by decreasing significance, with color indicating significance according to the legend’s color scale, the ratio of genes on the horizontal axis, and DEG count represented by circle size.
Fig 3.
Top 10 significant functional annotations of down-regulated DEGs.
(A) Biological processes, (B) cellular components, and (C) molecular functions, ranked by adjusted p-values. Functional annotations are ordered by decreasing significance, with color indicating significance level based on the legend’s color scale, the ratio of genes on the horizontal axis, and DEG count represented by circle size.
Table 1.
Description of functional annotations related to circadian rhythms when considering up-regulated DEGs.
Fig 4.
Network of functional annotations related to circadian rhythms for upregulated DEGs.
Nodes represent functional annotations, with node size indicating the number of genes within each Edges represent relationships between nodes, with edge thickness reflecting the strength of the association.
Fig 5.
Boolean network of circadian rhythms and their interplay with metabolism-related genes under normal diet conditions.
Nodes represent individual genes, with node size corresponding to the log-average of normalized transcriptome counts for each gene. Arrows indicate regulatory interactions, with their thickness representing the strength of influence from one gene to another.
Table 2.
Network properties of the Boolean network achieved.
Fig 6.
Simulation of the Boolean network on circadian rhythms under (A) normal diet and (B) high fat diet conditions.
Genes are shown on the vertical axis, with their states of evolution on the horizontal axis. The final states represent the attractors reached.