Fig 1.
(A): Solanum elaeagnifolium in lab, (B): Germination seeds on ½ MS medium.
Table 1.
The different treatments of growth regulators, NaCl and yeast extract supplemented to MS medium to test theireffect on alkaloid production.
Table 2.
Gene sequences.
Table 3.
Effect of the different ratio of growth regulator (BAP:2,4-D), with, and without NaCl and yeast extract supplemented to MS medium on callus diameter, color and softens of Solanum elaeagnifolium Cav.
Fig 2.
(A) Response of root and shoot of Solanum elaeagnifolium Cav. grown on MS medium supplemented with 1.0:1.0, mg L-1 of BAP: 2,4-D after2 weeks of Inoculation. (B,C) Response and callus formation of Solanum elaeagnifolium Cav on MS medium supplemented with 1.0:1.0, mg L-1 of BAP: 2,4-D (CGM1) after eight weeks of Inoculation. (D) Effect of NaCl (CGM3). (E) Effect of yeast extract (CGM4).
Table 4.
Alkaloid componentsidentified in Solanum elaeagnifolium leaf and callus cultures (CGM1–CGM4) by Liquid -chromatography–mass spectrometry.
Fig 3.
Chromatogram profiles of Solanum elaeagnifolium.
(A) Extract of leaves. (B) Extract of callus that growth on MS medium supplemented with 1:1 mg/L of BAP: 2, 4-D. (C) Extract of callus that growth on MS medium supplemented with 1.5:1.5 mg/L of BAP:2, 4-D. (D) Extract of callus that growth on MS medium supplemented with 1.5:1.5 mg/L of BAP: 2, 4-D + 1 g/L NaCl. (E) Extract of callus that growth on MS medium supplemented with 1:1 mg/L of BAP: 2, 4-D + 1.5 g/L Yeast.
Table 5.
Anticancer activities of Solanum elaeagnifolium alkaloids extracts on death of MCF7 Breast Cancer Cell Line after 24 h of treatment (IC50 ± SE).
Table 6.
Gene expression of MCF7 breast cancer cells treated with solanum elaeagnifolium callus extracts at 12 and 24 h.
Fig 4.
The influence of alkaloids extract (CGM1 treatment) on the expression of genes TP53, CDK1, BCL2, and BAX in MCF7 cells.