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Fig 1.

Flow chart of participants.

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Table 1.

Baseline characteristics of middle-aged and older adult by serum 25(OH)D concentration in NHANES 2001-2018.

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Table 2.

Hazard Ratios (95% CIs) for all-cause and cause-specific mortality according to serum 25(OH)D concentrations among participants with middle-aged and older adult in NHANES 2001–2018.

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Table 3.

Threshold effect analysis of serum 25(OH)D concentrations on all-cause and CVD mortality in middle-aged and elderly populations.

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Fig 2.

Dose–response associations of serum 25(OH)D level with risk of All-cause (a), CVD (b), Cancer (c) mortality.

The red solid line represents the estimated risk of all-cause and cause-specific mortality, with blue dashed lines showing 95% CI, Analyses were adjusted for Gender, Race, Education, Martial Status, BMI Group, Drinking, Smoking, CVD, Caloric intake, Physical activity, Stroke, Hypertension, Diabetes, and PIR. PIR, ratio of family income to poverty; BMI, body mass index; CVD, cardiovascular disease.

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Table 4.

Association between serum 25(OH)D and all-cause mortality according to subgroup.

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Fig 3.

Survival curves for all-cause mortality by weighted Kaplan-Meier method with Log Rank test.

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Fig 4.

Survival curves for cardiovascular disease mortality by weighted Kaplan-Meier method with Log Rank test.

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