Fig 1.
The experimental process of a rat DHCA model.
CPB: cardiopulmonary bypass; DHCA: deep hypothermic circulatory arrest.
Fig 2.
Heatmap and volcano plot of DEGs between the DHCA group and the control group.
(A) Heatmap of DEGs identified using the DESeq2 method between the DHCA group and the control group. (B) Volcano plot of DEGs identified using the DESeq2 method between the DHCA group and the control group.
Fig 3.
WGCNA and identification of significant modules.
(A) Dendrogram illustrating the clustering of 438 genes based on the topological overlap matrix (1-TOM). (B) Module-trait heatmap displaying clustered gene modules along with their correlation to genetic alteration in lungs following DHCA, including corresponding correlation coefficients and P values for each module. (C) Scatter plot indicating a significant positive correlation between the blue module and genetic alteration in lungs following DHCA.
Fig 4.
Functional enrichment analysis of candidate hub genes.
(A) GO enrichment analysis of the candidate hub genes. (B) KEGG pathway analysis of the candidate hub genes.
Fig 5.
Construction of PPI network and screening of hub genes.
(A) PPI network illustrating candidate hub genes. (B) The top eight hub genes identified utilizing the MCC algorithm from the CytoHubba plugin. (C) Differential mRNA expression levels of the top 8 hub genes between the DHCA group and control group in the sequencing data. (D) The transcription factors of hub genes predicted by iRegulon in Cytoscape. PPI: protein-protein interaction. MCC: Maximal Clique Centrality.
Fig 6.
Comparison of mRNA levels of hub genes between the DHCA and the control groups.
*P < 0.05, **P < 0.01, ****P < 0.0001.
Fig 7.
Comparison of HE staining and IHC staining of hub genes in lung tissues between the DHCA and the control groups.
(A) The results of HE staining in lung tissues between the two groups. (B-I) The analysis of IHC staining for hub genes in lung tissues was conducted between the two groups. The genes examined, in sequence, included FOS, FOSB, JUN, EGR1, ATF3, NR4A1, CCN1, and ZFP36. HE: hematoxylin and eosin; IHC: immunohistochemistry.