Fig 1.
Illustration of the linear setup of a canine cell-free hemoglobin (cfHb) solution processed by hemoperfusion using the Cytosorb® adsorber.
The cfHb solution is passed through the Cytosorb® adsorber of the Pure Adjust® (Nikkiso) blood purification platform and collected in a waste bag at a blood flow rate of 100 mL/min. Samples are collected at the sample collection port after the adsorber.
Fig 2.
Illustration of the circular setup of a canine cell-free hemoglobin (cfHb) solution processed by hemoperfusion using the Cytosorb® adsorber.
The cfHb solution is processed by the Pure Adjust® (Nikkiso) blood purification platform and the Cytosorb® adsorber and recirculated into the same storage bag, with a flow of 100 mL/min. Samples were collected at the collection ports before and after the adsorber.
Fig 3.
Illustration of the control setup: continuous circulation of canine cell-free hemoglobin (cfHb) solution without the Cytosorb® adsorber was performed.
The cfHb solution was processed using the Pure Adjust® (Nikkiso) blood purification platform and recirculated into the same storage bag with a flow rate of 100 mL/min for 24 hours.
Table 1.
cfHb volume, hemoglobin concentration, and hemoglobin reduction of canine cell-free hemoglobin (cfHb) solution undergoing hemoperfusion, applying the Cytosorb® adsorber in the linear and the circular setup and control run without the adsorber. The end hemoglobin concentration refers to the hemoglobin concentration in the waste bag after processing the whole cfHb solution. The dilutional effect of saline used for priming was already addressed during the calculation of the hemoglobin reduction.
Fig 4.
Hemoglobin concentration of three canine cell-free hemoglobin (cfHb) solutions undergoing hemoperfusion using the Cytosorb® adsorber with the linear setup, analyzed after the adsorber.
Fig 5.
Hemoglobin clearance of three canine cell-free hemoglobin (cfHb) solutions undergoing hemoperfusion using the Cytosorb® adsorber with the linear setup.
Fig 6.
Hemoglobin concentration of three canine cell-free hemoglobin (cfHb) solutions undergoing hemoperfusion using the Cytosorb® adsorber with the circular setup, analyzed before and after the adsorber.
In the circular setup, samples were taken more frequently for the first two L of processed cfHb solution. The scale on the x-axis is presented in different intervals, as most changes occurred in the first two L of processed cfHb solution.
Fig 7.
Hemoglobin clearance of three canine cell-free hemoglobin (cfHb) solutions undergoing hemoperfusion using the Cytosorb® adsorber with the circular setup.