Fig 1.
Single-cell transcriptome landscape of human nasal mucosa in control and CRS.
(A-B) UMAP plot of single-cell transcriptome profiles. Colors indicate clusters (A) and cell types (B). (C) Tissue prevalence of major cell clusters estimated by Ro/e score. (D) Heat map plot of marker genes in each cell types. (E) Heat map plot showing the most enrichment GO biological process terms of marker genes in each cell types. (F) Heat map plot of top5 transcription factors in each cell types.
Fig 2.
Analysis of metabolic differences in epithelial cell subtypes.
(A-B) UMAP plot of epithelial cell subtypes. Colors indicate clusters (A) and cell types (B). (C) Tissue prevalence of major cell clusters estimated by Ro/e score. (D) Dot plot of top5 marker genes in each epithelial cell subtypes. (E) Dot plot showing specific metabolic pathway score in different epithelial cell subtypes. (F) Volcano plot showing the differential metabolic pathways in secretory cells. (G) Box plot showing the score of Nitrongen metabolism and Thiamine metabolism. *** p < 0.001.
Fig 3.
CD4+ Th2 cells are specific in eCRSwNP.
(A) UMAP plot of CD4+ T cell subtypes. Colors indicate cell types. (B) Dot plot of top5 marker genes in each CD4+ T cell subtypes. (C) Tissue prevalence of major cell clusters estimated by Ro/e score. (D) Bar plot showing the most enrichment GO biological process terms of marker genes in CD4+ Th2 cells. (E) Venn plot showing the overlapped genes of CD4+ Th2 markers and ferroptosis genes. (F) Violin plot of PPARG and SOCS1 in CD4+ T cell subtypes.
Fig 4.
Communication between DCs and CD4+ T cells.
(A) UMAP plot of DC cell subtypes. Colors indicate cell types. (B) Dot plot of top10 marker genes in each DC cell subtypes. (C) Tissue prevalence of major cell clusters estimated by Ro/e score. (D) Signaling pathways with significant differences in the overall information flow of each sample groups. (E) Differential number of interactions in DCs and CD4+ T cells. (F) Chord diagram showing up-regulated ligand-receptor pairs in DCs and CD4+ T cells. (G) Violin plot showing expression of ligand-receptor pairs in DCs and CD4+ T cells.
Fig 5.
Developmental trajectory of macrophages.
(A-C) UMAP plot of macrophage subtypes. Colors indicate clusters and cell types. (D) Tissue prevalence of major cell clusters estimated by Ro/e score. (E) Dot plot of marker genes in each macrophage subtypes. (F) Pseudo-time plots of macrophage. Color-coded according to states, clusters and pseudo-time value. (G) Heat map showing of differential pseudo-time genes. (H) Line chart showing the C1QA, C1QB, C1QC, HRH1 and FN1 relative expression according by pseudo-time. (I) Bar plot of pseudo-time states in different groups. (J) Dot plot of top5 marker genes in each pseudo-time states. (K) Violin plot of S100A8, CCL18 and EGER in macrophage subtypes.