Fig 1.
Plasma GSH level positively predicts stereotypy severity in the young group (n = 10; 140-165 days old), but not in the old group (n = 9; 180-225 days old), of C57BL/6 mice.
Data are corrected for blocking factors in the analysis. Circles on the graph depict individual mice. Lines on the graph depict regression lines for the two age groups.
Fig 2.
Protein expression predicts both plasma GSH level and stereotypy severity in n = 18 C57BL/6 mice (n = 10 young mice; n = 9 old mice).
Regression slope + /- standard error. a) expression of nine proteins significantly correlated with GSH level as a function of age. The light gray shading depicts proteins that pass BH correction at critical q = 5%; medium gray denotes proteins that pass BH correction at critical q = 10%; dark gray shading depicts proteins that pass BH correction at 20%. Orange bars depict protein expression in the young group of mice and blue bars depict protein expression in the old group of mice. ‡ denotes proteins that pass BH correction at critical q = 5% within age group; † denotes protein that passes BH correction at critical q = 20% within age group. b) Expression of 15 proteins correlated with stereotypy severity controlling for age. The light gray shading depicts proteins that pass BH correction at critical q = 5%; medium gray shading denotes proteins that pass BH correction at critical q = 10%; dark gray shading depicts proteins that pass BH correction at 20%.
Fig 3.
Expression of proteins significantly correlated with stereotypy severity in CD1 Mice (n = 28).
Regression slope + /- standard error. Expression of 7 proteins significantly correlated with stereotypy severity in CD1 mice. Light gray shading denotes protein passed BH correction with critical q = 5%; darker gray shading denotes protein passes BH correction at critical q = 20%. Expression of Pdgfb, Riox2, Parp1, Snap29, Map2k6, and Cxcl9 increase with severity of stereotypy, whereas expression of Vsig2 decreases with stereotypy severity. Expression of Pdgfb, Riox2, and Parp1 is also correlated with plasma GSH level and stereotypy severity in C57BL/6 mice, indicating that they are biomarkers of REDOX balance and stereotypy severity.
Fig 4.
Ontology of identified proteins which passed BH correction of q < 20% in at least one analysis in either the C57BL/6 (n = 18) or CD1 mice (N = 28).
a) Venn diagram depicting proteomic “hit” associations with REDOX physiology (far right section), dopamine physiology (far left section), or both REDOX and dopamine physiology (middle section). b) Venn diagram depicting association of proteomic “hits” as biomarkers for three disorders (ASD, Schizophrenia, and ADHD). Proteins associated with multiple disorders are located in overlapping region(s) enclosed by disorder specific circles. Proteins located outside of the Venn diagram are not associated with any of these disorders.