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Fig 1.

Experimental design and the different feeding conditions.

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Fig 2.

(A) Relative Candida tropicalis load in the gut of BSF larvae from different treatments: supplemented C. tropicalis throughout the rearing process – CT + ; supplemented C. tropicalis throughout the rearing process except for the last day – CT-; no supplemented C. tropicalis – control). (B) relative C. tropicalis load in different gut regions (anterior midgut – AM, middle midgut – MM, posterior midgut – PM, and hindgut – H). The values represent averages with standard deviation of gene 26S expression. Significant differences (p < 0.05) are marked by different letters above the bars and error bars represent standard error.

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Fig 3.

Percent (in weight) of the different fatty acids (represented by the numerical symbol of the total amount of carbon atoms of the fatty acid and the number of double bonds) related to total fatty acids in Candida tropicalis.

Error bars represent standard error.

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Table 1.

Metabolites and pathways that increased in BSF larvae fed with Candida tropicalis compared to larvae without any supplemented fungi in the substrate.

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Table 2.

Metabolites and pathways that increased in the biomass of Candida tropicalis relative to other fungi (Saccharomyces cerevisiae).

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Table 2 Expand

Table 3.

Metabolites and pathways that increased in the BSF rearing substrate supplemented with Candida tropicalis compared to substrate without fungi.

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Table 3 Expand

Fig 4.

Lysozyme (A) and total proteolytic (B) activity in the gut of BSF larvae fed with supplemented Candida tropicalis (Ct) or without supplemented C. tropicalis (Control).

Significant differences (p < 0.05) are marked by different letters above the bars; error bars represent standard error.

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Fig 4 Expand