Fig 1.
The structures of thionated levofloxacin derivatives.
Table 1.
Cytotoxicity (IC50 ± SD) of DOX and compounds 2 and 3 on PC-3, MCF7, Caco-2, H69, and H69AR cell lines after treatment for 96 h; n = 3.
Fig 2.
Cell viability assays using compound 3 on PC-3 cell line alone at various concentrations (μM) (dark grey bars) and in combination with DOX (light grey bars) at or around DOX’s IC50 value on the cell line.
No treatment is presented with control bar chart (black bar). White bar is DOX at or around its IC50 value alone. Experiments were conducted in triplicates at three independent trials with controls (*** p < 0.001, and **** p < 0.0001).
Fig 3.
Cell viability assays using compound 3 on MCF7 cell line alone at various concentrations (μM) (dark grey bars) and in combination with DOX (light grey bars) at or around DOX’s IC50 value on the cell line.
No treatment is presented with control bar chart (black bar). White bar is DOX at or around its IC50 value alone. Experiments were conducted in triplicates at three independent trials with controls.
Fig 4.
Cell viability assays using compound 3 on Caco-2 cell line alone at various concentrations (μM)(dark grey bars) and in combination with DO X (light grey bars) at or around DOX’s IC50 value on the cell line.
No treatment is presented with control bar chart (black bar). White bar is DOX at or around its IC50 value alone. Experiments were conducted in triplicates at three independent trials with controls.
Fig 5.
Cell viability assays using compound 3 on H69 cell line alone at various concentrations (μM) (dark grey bars) and in combination with DOX (light grey bars) at or around DOX’s IC50 value on the cell line.
No treatment is presented with control bar chart (black bar). White bar is DOX at or around its IC50 value alone. Experiments were conducted in triplicates at three independent trials with controls (**** p < 0.0001).
Fig 6.
Cell viability assays using compound 3 on H69AR cell line alone at various concentrations (μM) (dark grey bars) and in combination with DOX (light grey bars) at or around DOX’s IC50 value on the cell line.
No treatment is presented with control bar chart (black bar). White bar is DOX at or around its IC50 value alone. Experiments were conducted in triplicates at three independent trials with controls (*** p < 0.001, and **** p < 0.0001).
Table 2.
IC50 value (µM ± SD) for compound 3 as single agent and in combination with DOX and for that of DOX as single agent and in combination with compound 3, along with the calculated combination indices for the combination treatments on PC-3, H69, and H69AR cell lines after treatment for 96 h; n = 3.