Fig 1.
Therapeutic effects of Nintedanib on acute radiation-induced lung injury in mice.
(A) Pathological changes in lung tissues of mice in each group (HE staining 200×). (B) Lung tissue szapiel score of mice in each group(Compared with the control group, *P ＜ 0.05; Compared with the radiotherapy group, # P ＜ 0.05; Compared with the low dosage group, △P ＜ 0.05).(C)Immunohistochemical staining for the expression of Smad2 in lung tissues(200×).(D) The percentage of Smad2 positive expression area (Compared with the control group, *P ＜ 0.05; Compared with the radiotherapy group, # P ＜ 0.05; Compared with the low dosage group, △P ＜ 0.05).
Fig 2.
Long-term protective effects of Nintedanib on chronic radiation-induced lung injury in mice.
(A) The chest hair condition of mice in each group at 3 months after radiotherapy;Pathological changes in lung tissues of mice in each group (Masson staining 200×);Immunohistochemical staining for the expression of Smad2 and αSMA in lung tissues(200×).(B) The change trends of mice body weight. (C) Lung tissue ashcroft score of mice in each group(Compared with the control group, *P ＜ 0.05; Compared with the radiotherapy group, # P ＜ 0.05; Compared with the low dosage group, △P ＜ 0.05). (D) The percentage of Smad2 and αSMA positive expression area(Compared with the control group, *P ＜ 0.05; Compared with the radiotherapy group, #P ＜ 0.05; Compared with the low dosage group, △P ＜ 0.05).
Table 1.
Level of IL-6, TNF-α and TGF-β1 in the serum of mice in each group (‾X ± S, pg/mL, N = 10).
Table 2.
Level of TGF-β1 in the serum of mice in each group (‾X ± S, pg/mL, N = 10).