Table 1.
Top 10 proteins differentially expressed in brain samples from dogs infected with rabies virus (vs. brain samples from dogs without rabies virus).
Fig 1.
Gene ontology analysis results for the proteomic and phosphoproteomic data.
A. Results obtained using the proteomic data. B. Results obtained using the phosphoproteomic data.
Table 2.
Top 10 proteins differentially phosphorylated in brain samples from dogs infected with rabies virus (vs. brain samples from dogs without rabies virus).
Table 3.
Differentially expressed and phosphorylated proteins involved in the synaptic vesicle cycle.
Fig 2.
The analysis of protein-protein interaction network from proteomic results.
The results of the STRING analysis performed with the differentially expressed proteins. The red circles indicate proteins involved in the synaptic vesicle cycle.
Fig 3.
The differentially expressed and phosphorylated proteins associated with the synaptic vesicle cycle.
The synaptic vesicle cycle, adapted from the Kyoto Encyclopedia of Genes and Genomes database (pathway entry: cfa04721), and its five steps: docking (1), priming (2), fusion (3), full fusion (4), and endocytosis (5). The red rectangles indicate differentially expressed proteins, and the blue rectangles indicate differentially phosphorylated proteins.
Fig 4.
Identification of kinases involved in the phosphorylation of RABV phosphoprotein, synaptotagmin-1, and syntaxin-binding protein 1.
A. Schematic diagram showing the phosphorylation sites and their corresponding kinases. B. Pie chart showing the percentage of phosphorylation sites on the target proteins associated with each kinase.
Fig 5.
Rabies virus production in kinase inhibitor-treated Neuro-2a cells at three time points.
Data are shown as mean ± standard error. * p < 0.05. hpi: hours post-infection.