Table 1.
Case overview.
Fig 1.
Morphology of marmoset dorsal lateral geniculate nucleus (DLG).
(A) Digital photomicrographs of a thionin (Nissl substance) stained coronal sections through DLG, showing external parvocellular (PE), internal parvocellular (PI), internal magnocellular (MI), external magnocellular (ME) and koniocellular (K1–K4) layers. Scale bar: 500 µm. Abbreviations: IP, inferior pulvinar; RT, reticular nucleus.
Table 2.
Mean, confidence intervals (95%) and observed difference for each factor.
Fig 2.
Photomicrographs of CB-immunostained histological sections containing the marmoset dorsal lateral geniculate nucleus (DLG).
(A) Representative sections of the DLG used in the present analysis. (B-G) CB immunoreactivity in the DLG of adult (left) and aged (right) animals at rostral (B-C), middle (D-E) and caudal (F-G) levels. Scale bar: 500µm. Abbreviations: IP, inferior pulvinar; K1, koniocellular layer 1; K3, koniocellular layer 3; K4, koniocellular layer 4; PE, external parvocellular layer; PI, internal parvocellular layer; RT, reticular nucleus. Adapted from Paxinos et al. (2012).
Fig 3.
Stereological estimations of morpho-quantitative parameters in marmoset dorsal lateral geniculate nucleus (DLG).
(A and B) Dot plot illustrates the distribution and statistical comparison of age groups for (A) CB+ neuronal number and (B) CB+ somal volume (in µm3). The permutation test shows no significant difference for CB+ neuronal count (p = 0.06571) and CB+ cellular volume (p = 0.19954) between adult (n = 4) and aged animals (n = 2). For this plot, each point represents a mean difference between the permuted data. These findings was further corroborated by (C) Spearman (ρ = 0.65, p = 0.17) and (D) (ρ = -0.37, p = 0.49) correlation tests, which indicated CB+ neuronal number and CB+ somal volume do not vary significantly with age, respectively. For correlation data, each point represents one individual. Significance at p > 0.05.
Fig 4.
High magnification photomicrographs of CB-immunolabeled DLG neurons.
(A–D) CB immunoreactivity in the koniocellular strata of adult (left) and aged (right) animals, specifically in K1 and K3 layers, respectively. Black arrowheads point to neurons with an evident CB immunostaining and punctate cell-like morphology, while white arrowheads indicates cells excluded from the counting due to weak immunolabeling and other morphological shapes. Scale bar: 50µm (A–D).