Fig 1.
Right atrial appendage tissue was collected from nine patients. Tissues were kept at varying temperatures (4°C or 22°C) and time intervals (zero (reference), one, seven, 14, or 28 days) before RNA extraction and subsequent sequencing. All combinations of temperatures and times were investigated in duplicate. Created with Biorender.com.
Fig 2.
Proportion of protein-coding, mitochondrial (mtRNA), and long non-coding RNAs (lncRNA) of all gene-annotated sequencing reads from tissues stored at varying time intervals and temperatures.
Fig 3.
Principal component analysis (PCA) of global gene expression profiles. A) PCA plot of all samples stratified by time and temperature. B) Variance explained by principal components (PCs) 1–10. C) Association analysis of PCs 1–10 and variables. R2 = Squared Pearson correlation coefficient, * = p-value < 0.05, ** = p-value < 0.01, *** p-value < 0.0001.
Table 1.
Number of reported differentially expressed genes in paired tissues stored for one, seven, 14, and 28 days at 4°C and 22°C prior to RNA extraction. Pairwise differential expression analysis was performed using day zero as the reference. A) The reported number of differentially expressed genes without including covariates in the model. B) The reported number of differentially expressed genes when including RNA integrity number (RIN) as a covariate. Percentages are of the total number of tested genes (n = 58,601). False discovery rate < 5% was used.
Fig 4.
Overlap in genes classified as differentially expressed in paired tissues stored at different time intervals before RNA extraction.