Fig 1.
SERS spectra for blood from rats injected with GH only, TE only, both GH and TE hormones, and non-injected rats.
Fig 2.
Three-dimensional PCA (a) score plots, (b) loadings plot for (i) PC1, (ii) PC2, and (iii) PC3 loadings plot, and averaged SERS spectra of blood from (iv) rats injected with GH only, (v) rats injected with TE only and (vi) non-injected rats.
The explained variances are indicated in percentages and were 43.2%, 23.2%, and 15.1% for PC1, PC2, and PC3 respectively.
Fig 3.
SERS spectra of different for blood from (a) non-injected rats, (b) rats injected with GH only, (c) rats injected with TE only, and (d) rats injected with both GH and TE hormones.
The blood was drawn at different times before and after injection.
Fig 4.
Three-dimensional PCA score plots for (a) rats injected with GH only, (b) Normal rats, (c) rats injected with TE only, and (d) rats injected simultaneously with GH and TE based on different times before and after injection.
Fig 5.
PCA loading plots and SERS Spectra of blood from rats injected with (a) GH only, (b) non-injected rats, (c) rats injected with TE only, and (d) rats injected simultaneously with GH and TE hormones drawn at different times before and after injection.
Table 1.
Validation metrics of the ANN models constructed, trained and validated.
Fig 6.
ANN models’ regression curves showing predicted concentration against actual concentration.
Table 2.
The comparison between the detection limits for the ANN models created in this work with those published in the literature.
Fig 7.
Predicted concentrations using the (a) GH and (b) TE ANN models.
Fig 8.
ELISA results for non-injected rats and rats injected singly and simultaneously with GH and TE hormones.