Table 1.
Specifications of commercial GRIN lenses.
Table 2.
Suggested GRIN lenses for various brain region imaging.
Fig 1.
Lens holders for different sizes of GRIN lenses.
(A) Homemade vacuum lens holders for GRIN lenses with 1 mm and 1.8 mm diameters. (B) Commercial lens holders for GRIN lenses with 1 mm diameters. (C) Lens holders for GRIN lenses with 0.5 mm and 0.6 mm diameters.
Fig 2.
Instructions for homemade lens holders.
(A) Diagram of homemade lens holder using two pipette tips. (B) Tools for making lens holders. (C) Instruction for making a 1.8 mm lens holder. 1, Cut 4.5 mm from the tip of METTLER TOLEDO 200 μl pipette tip and remove the filter. Cut the back of the same pipette tip to make it fit 1 ml syringe. 2, Cut 5 mm from the tip of Eppendorf 20 μl pipette tip and cut the back at where the diameter significantly changes. 3 and 4, Insert the thinner cut tip into the thicker one and push it to the end. 5, Test. (D) Instruction for making a 1 mm lens holder. 1, Cut 3.5 mm from the tip of METTLER TOLEDO 100 μl pipette tip and remove the filter. Cut the back of the same pipette tip to make it fit 1 ml syringe. 2, Cut 11 mm from the tip of Fisher scientific 10 μl pipette tip and cut the back approximately at where the diameter significantly changes. 3 and 4, Insert the thinner cut tip into the thicker one and push it to the end. 5, Test. (E) Instruction for making a 0.5 mm lens holder. 1, Cut 1 mm from the tip of METTLER TOLEDO 100 μl pipette tip and remove the filter. Cut the back of the same pipette tip to make it fit 1 ml syringe. 2, Cut 2 mm from the tip of Fisher scientific 10 μl pipette tip and cut the back approximately at where the diameter significantly changes. 3 and 4, Insert the thinner cut tip into the thicker one and push it to the end. 5, Test. (F) Example of the vacuum holder system.
Fig 3.
Aspiration system. (A) Aspiration system is composed of a flask, the wall vacuum port, venous infusion needle and a 1 ml syringe. (B) Drill a hole on the side of the syringe for controlling vacuum by finger pressing.
Table 3.
Surgical instruments information.
Table 4.
Supplies and reagents for lens implantation surgery.
Fig 4.
Diagram of different surgical procedures. (A) Procedure for the implantation of large/medium size lenses. (B) Procedure for the implantation of small diameter lens with partial aspiration. (C) Procedure for the implantation of small diameter lens without aspiration.
Fig 5.
Raw imaging, extracted cells and activity traces.
(A–C) Calcium imaging recorded in NAc through a 0.6 mm in diameter and 7.3 mm long relay lens by Miniscope V4, 154 neurons. (D–F) Calcium imaging recorded in PrL through a 1 mm in diameter and 4 mm long relay lens by Miniscope V4, 536 neurons. (G–I) Calcium imaging recorded in in IL through a 0.5 mm in diameter and 6.1 mm long relay lens by Miniscope V4, 193 neurons. (J–L) Calcium imaging recorded in DP through a 0.5 mm in diameter and 6.1 mm long relay lens by Miniscope V4, 151 neurons. (M–O) Calcium imaging recorded in dCA1 through a 1 mm in diameter and 4 mm long relay lens by Miniscope V4, 279 neurons. (P–R) Calcium imaging recorded in vCA1through a 0.5 mm in diameter and 6.1 mm long relay lens by Miniscope V4, 66 neurons. (S–U) Calcium imaging recorded in CA2 through a 1 mm in diameter and 4 mm long relay lens by Miniscope V3, 97 neurons. All Left panels, raw calcium fluorescence frames of the field of view (FOV) from example animals; All middle panels, single neurons extracted from the FOV; All right panels, calcium traces of ten example cells recorded from these brain regions. Mice were six to eight weeks old when surgeries were performed.
Fig 6.
Multi-region imaging using MiniXL.
(A–E) Bilateral mPFC calcium imaging through 1 mm in diameter and 4 mm long relay lenses by MiniXL. (F–J) PFC & NAc calcium imaging through 0.5 mm in diameter and 6.1 mm long relay lens (PFC) and 0.5 mm in diameter and 8.4 mm long relay lens by MiniXL. A and F, raw calcium fluorescence frames of the field of view (FOV) from example animals; B, D, G and I, single neurons extracted from the FOV; C, E, H and J, calcium traces of ten example cells. Mice were six to eight weeks old when surgeries were performed. More details can be found in S1 Table.