Fig 1.
Representative R/G signals (Red spots/Green spots) for normal status
(A), deletion status without polysomy (B) and deletion status with polysomy (G ≥ 3) for 1p/1q and 19q/19p (C).
Table 1.
Clinical, histological and molecular data according to the tumor grade and the polysomic status.
Fig 2.
Event Free Survival (EFS) and Overall Survival (OS) according to the histological grade and to the chromosomal status. O3 anaplastic oligodendrogliomas are associated with shorter EFS and OS (raw 1). The presence of a CDKN2A gene deletion on 9p (both homozygous and heterozygous deletion) is associated with a shorter OS and a trend to a shorter EFS (raw 2).
Table 2.
Correlation between clinical, histological and molecular data with survival in univariate analysis.
Fig 3.
Event Free Survival (EFS) and Overall Survival (OS) according to the 1p and/ or 19q polysomic status.
Polysomy is associated to a shorter EFS and OS (raw 1) regardless to 1p/19q copolsomy or single 1p or 19q polysomy status (raw 2). Polysomy is strongly associated to histological high grade (raw 3) and within the O3 cohort, presence of polysomy still corelated to shorter EFS and OS (raw 4).
Fig 4.
Forest plots of Cox proportional hazard regression analysis for EFS and OS in univariate and multivariate analysis of hazard ratios (HR) and 95% confidence intervals (CI) of features in our cohort.
In the multivariate model, polysomy is independently associated with significantly shorter delay before recurrence (EFS) and worse clinical outcome (OS).
Table 3.
Concordance between manual and automated analysis in the determination of 1p and 19q polysomy status.
Fig 5.
Repartition of R/G signals with 1p/1q and 19q/19p probes obtained by automated FISH analysis.
A,B: global R/G signal repartition with signals ≤ 0,75 corresponding to deletion status, between 0,75 and 1 to instability status, = 1 to normal status and > 1 to polysomy without deletion status. C,D: R/G signal repartition within the deletion + polysomy cohort revealed 4 distinct subgroups of signals corresponding to homozygosity for the chromosome (R/G = 0), hemizygosity (R/G < 0,5), polyploidy (R/G = 0,5 with an exception for the 1/2 signature) and instability (R/G > 0,5 and ≤ 0,75). E,F: significant R/G signatures in deletion + polysomic cohort identified 7 major signatures for both Chromosome 1 and 19 probes with a major predominance of R/G = 1/3, 2/4 and 2/3 signatures.