Fig 1.
Hemocytes distribution in the adult fly.
Fluorescence image of Hml-Gal4 > UAS-RFP flies showing the distribution of hemocytes in the thorax and abdomen. Magnification 10X.
Fig 2.
Illustration of the technique.
Schematic representation of the protocol for plasmatocyte isolation from hemolymph of adult flies.
Fig 3.
Plasmatocytes stained with crystal violet.
Bright field images of plasmatocytes (a-a’) isolated from the hemolymph of adult fly, stained with crystal violet showing nucleus and cell membrane. Magnification 100X.
Fig 4.
Phagocytic activity of plasmatocytes.
Images of phagocytic activity of plasmatocytes a) Bright field image of plasmatocytes showing phagocytosis at 40X magnification b) Fluorescence images of plasmatocytes exhibiting phagocytosis at 100X magnification. Red = PI (Propidium Iodide) stain and Blue = DAPI. Please note that phagocytosis is specific to the cytoplasmic region of plasmatocytes.
Fig 5.
Bright field images of plasmatocytes on the hemocytometer grid (a) Immediately after loading the cells on the hemocytometer, all cells (100%) were found to be viable (a’) after 2 hours of incubation, 42% of the cells incorporated Trypan blue and appeared blue, indicating cell death. Representative images are captured using a 10X objective lens and zoomed to 0.76X, resulting in a total magnification of 7.6X.
Fig 6.
P1 antibody staining of plasmatocytes.
Fluorescence images of different regions (a-a’) showing localization of NimC1 protein, a plasmatocyte-specific marker (P1antibody). Red = P1 protein and Blue = DAPI. Magnification 20X.