Fig 1.
Three frozen tissue samples were collected from each of 10 individuals of 10 marine species (five marine fishes and five marine invertebrates) for a total of 300 samples. One sample from each individual was then thawed in EDTA (250 mM, pH 10) overnight at 4°C and the second was thawed in ethanol (95%) overnight at 4°C, after which DNA was extracted, analyzed, and compared to DNA extracted directly from the third frozen tissue sample, which did not receive liquid preservative treatment.
Table 1.
The effect of preservative treatments on average percentages of high molecular weight DNA (%HMW) and average yields of high molecular weight DNA normalized by tissue weight (nY) for DNA extracts from frozen tissues of 10 specimens of each of 10 marine species.
Fig 2.
Percentage of high molecular weight DNA.
Average values for percentage of high molecular weight DNA (%HMW) were determined for extracts prepared from frozen tissue samples collected from each of 10 individuals of 10 marine species (five marine fishes and five marine invertebrates). One sample from each individual was then thawed in EDTA (250 mM, pH 10; maroon) overnight at 4°C and the second was thawed in ethanol (95%; blue) overnight at 4°C, after which DNA was extracted, analyzed, and compared to DNA extracted directly from the third frozen tissue sample, which did not receive liquid preservative treatment (yellow). Error bars represent standard error. Within each histogram, treatments bearing different lower-case letters are significantly different at p < 0.05; matching lower-case letters indicate statistically indistinguishable treatments; an absence of letters indicates no significant differences among all treatments in a given model.
Fig 3.
Normalized yield of high molecular weight DNA.
Average values for yields of high molecular weight DNA normalized by tissue weight (nY) were determined for extracts prepared from frozen tissue samples collected from each of 10 individuals of 10 marine species (five marine fishes and five marine invertebrates). One sample from each individual was then thawed in EDTA (250 mM, pH 10; maroon) overnight at 4°C and the second was thawed in ethanol (95%; blue) overnight at 4°C, after which DNA was extracted, analyzed, and compared to DNA extracted directly from the third frozen tissue sample, which did not receive liquid preservative treatment (yellow). Error bars represent standard error. Within each histogram, treatments bearing different lower-case letters are significantly different at p < 0.05; matching lower-case letters indicate statistically indistinguishable treatments; an absence of letters indicates no significant differences among all treatments in a given model. Note that y-axis scales differ among species.