Fig 1.
Timeline of Solitary or Social Partner PMA conditioning.
Fig 2.
Solitary and Social Partner PMA apparatuses.
(A) Conditioning chamber setup for Solitary PMA and (B) Social Partner PMA. The right side of the Social Partner PMA chamber is symmetrical to the left side. (1) LED house light; (2) USB wide-angle camera; (3) Speaker; (4) Pellet dispenser; (5) Lever; (6) Food dish; (7) Platform; (8) Shock floor grid; (9) Drop pan. All measurements are in cm.
Fig 3.
Zone designation and corresponding heat maps in ANY-Maze.
(A) Schematic of zones and operant landmarks in Solitary PMA. (B) Example heat map of location averaged across all animals from a single cohort in Solitary PMA conditioning (Male n = 10 and Female n = 6). (C) Schematic of zones and operant landmarks in Social Partner PMA. (D) Example heat map of location averaged across all animals from a single cohort in Social Partner PMA conditioning (Male n = 5 and Female n = 4).
Fig 4.
Time-course of behaviors during PMA conditioning.
(A-B) Avoidance, (C-D) freezing, (E-F) lever-pressing behaviors at Days 1 (light colored lines), 3 (dark colored lines), and 10 (black lines) of PMA conditioning under Solitary (n=49; 20 females, 28 males) and Social Partner (n=46; 23 females, 23 males) contexts. The first tone period from each session is indicated by the gray box. *p<0.05, **p<0.01, ***p<0.001. See Data for Fig 4 file in Supporting Information for data used to generate this figure.
Table 1.
Operant box and equipment needed for Social Partner and Solitary PMA tasks.
Table 2.
Additional operant box and equipment needed only for Social Partner PMA task.
Table 3.
Troubleshooting notes for behavioral training during PMA tasks.