Table 1.
Database and analysis tools.
Fig 1.
Research flowchart.
Fig 2.
Venn diagram and protein–protein interaction (PPI) networks.
(A) Venn diagram: red represents ischemic stroke, blue represents ferroptosis, and purple represents intersecting targets. (B) PPI networks of 27 potential targets associated with ischemic stroke in ferroptosis. The intensity of the color corresponds to the degree value, with darker colors indicating higher values.
Fig 3.
Screening hub genes by machine learning.
(A) LASSO regression algorithm. (B) RF algorithm. (C) SVM algorithm. (D) Venn diagrams for the three algorithms. LASSO, Least Absolute Shrinkage and Selection Operator; SVM, Support Vector Machine; RF, Random Forest.
Table 2.
Results of the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of 14 core targets.
Fig 4.
Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses.
Table 3.
Top 10 compounds ranked by degree value in the target-compound interaction network.
Fig 5.
Receiver operating characteristic curve analysis.
Hub genes in the GSE58294 dataset were analyzed using the receiver operating characteristic curves.
Fig 6.
Visualization network of potential target and compound matching.
Red nodes represent targets, and blue nodes represent compounds. The top 10 compounds are highlighted on the right side of the network diagram.
Table 4.
Top 10 compounds ranked by degree value in the targets-compounds-TCM network.
Table 5.
Top 10 Chinese medicines ranked by degree value.
Fig 7.
Visualization of the target-compound-TCM network.
The red nodes represent the target, the blue nodes represent the compound, and the green nodes denote the TCM. At the center of the network diagram, the TCMs and small molecules with the highest correlations are highlighted.
Fig 8.
Properties, tastes, meridian tropism, and efficacy analysis of the candidate TCMs.
Table 6.
Docking information of the small molecules.
Table 7.
Target docking information.
Fig 9.
Molecular docking heat map of the core target interactions with the compounds.
Fig 10.
Docking results of the primary target related to ferroptosis in IS and its interaction with TCM compounds.
Fig 11.
Ferroptosis mechanism illustration.