Fig 1.
Experimental flow chart of the study.
Fig 2.
OF2 isolate phylogenetic tree generated by using MEGA 11 software related to B. amyloliquefaciens.
Table 1.
GC-MS results of the 23 secondary metabolites present in the extract of OF2 isolate, identified using GC-MS QP2010 Plus (Shimadzu, Japan).
Fig 3.
The UV-VIS spectrophotometric analysis of AgNPs, synthesized by using OF2 secondary metabolites extract, revealed distinct Plasmon resonance peaks at 420nm.
Fig 4.
SEM micrograph of AgNPs at 1000x magnification (A) and EDS elemental spectrum of biosynthesized AgNPs (B).
Fig 5.
The FTIR spectrum (A) and Zeta potential (B) of AgNPs synthesized by using OF2 secondary metabolites extract.
Table 2.
Antibacterial activity of OF2 secondary metabolites extract and OF2-AgNPs determined by agar well diffusion method at 2 mg/mL concentration (S1 Table).
Fig 6.
DDPH radicals scavenging activity (%) of B. amyloliquefaciens derived secondary metabolites (OF2) and their synthesized AgNPs (OF2-AgNPs), ascorbic acid was used as a reference compound.
The statistical significance level represents *p<0.05 and **p<0.01 (S2 Table).
Fig 7.
The latency time (seconds) of OF2 secondary metabolites extract and its mediated AgNPs at various concentrations, diclofenac sodium was used as standard (Std) while normal saline (NS) as a control.
The statistical significance level represents *p<0.05, **p<0.01, and ***p<0.001 (S4 Table).