Table 1.
Influence of different sucrose and PGRs level on growth parameters at multiplication and elongation stage.
Fig 1.
Effect of explants on establishment of cultures on MS media supplemented with 1.0 mg/L BAP.
(A) shoot tip cultures. (B) axillary bud cultures.
Fig 2.
Effect of BAP concentrations in MS media on shoot induction from shoot tip and axillary bud explants.
(A) percentage explants induced shoot buds out of 25 shoots. (B) mean length (cm) of shoot buds eight weeks after inoculation (2nd subculture). (C) mean number of shoot buds 16 weeks after inoculation (4th subculture).
Fig 3.
Growing axillary bud cultures (at 16 weeks, at the end of 4th subculture) on MS media supplemented with different concentrations of BAP.
(A) 0 BAP. (B) 1.0 mg/L BAP.(C) 2.0 mg/L BAP.(D) 5.0 mg/L BAP.
Fig 4.
Elongation and multiplication of shoots on MS media supplemented with 40 g/L of sucrose,1.0 mg/L BAP and 0.1 mg/L IBA.
(A) at the time of transferring (16th week of establishment). (B) at the end of 1st subculture (20th week of establishment). (C) at the beginning of 2nd subculture (shoots cluster separated). (D) multiplication of shoots at the end of 2nd subculture (at the end of 24th week of establishment). (E)one cluster of multiplied shoots at the end of 24th week.
Table 2.
Effect of ½ MS media supplemented with different concentration of sucrose and IAA in root induction.
Fig 5.
In vitro rooted plantlets in½ MS media supplemented with 40 g/L sucrose and 1.0 mg/L IAA.
Table 3.
Effect of pulsing treatments of shoots using 1000 ppm IAA and IBA for different pulsing period on ex vitro rooting.
Fig 6.
Rooted shoots on sand medium with different treatments.
(A) 1000 mg/L IAA for 15 minutes. (B) 1000 mg/L IAA for 30 minutes. (C) 1000 mg/L IBA for 15 minutes. (D) 1000 mg/L IBA for 30 minutes.(E) control.
Fig 7.
Plants rooted on sand medium at different stages of growth.
(A and B) Plants during acclimatization. (C) in vitro propagated one year old G. walla plants.
Fig 8.
TLC fingerprints developed for comparing in vitro grown plantlets with agarwood of G. walla under 15% methanol: 85% chloroform solvent system (v/v).
(A) in vitro plantlets (TC). (B)agarwood of G. walla (Ag).