Fig 1.
A diagram indicating the hemolytic and non-hemolytic activities of certain Bacillus sp. isolates.
(A) represents the hemolytic diagram of the strain. (B) represents the non-hemolytic diagram of the strain.
Fig 2.
Drug sensitivity analysis of 34 Bacillus sp. isolates to 16 antibiotics.
Fig 3.
PCR amplification of antibiotic resistance genes in denitrifying Bacillus sp. isolates.
(A) electrophoresis schematic diagram of tetB gene, lane M indicates the molecular marker (2000 bp), lanes 1-24 indicate the tetB positive strains (206 bp). (B) electrophoresis schematic diagram of cfr gene, lane M indicates the molecular marker (2000 bp), lane 34 indicates the cfr positive strain (746 bp). (C) electrophoresis schematic diagram of blaTEM gene, lane M indicates the molecular marker (2000 bp), lane 28 indicates the blaTEM positive strain (425 bp).
Fig 4.
PCR amplification results of three functional genes from 34 Bacillus sp. isolates.
(A and B) Gel electrophoresis of naP in 34 Bacillus sp. isolates (2040 bp). Lane M: molecular marker (2000 bp). (C and D) Gel electrophoresis of nor in 34 Bacillus sp. isolates (1917 bp). Lane M: molecular marker (2000 bp). (E and F) Gel electrophoresis of narG from 34 Bacillus sp. isolates (256 bp). Lane M: molecular marker (2000 bp).
Table 1.
The specific activity of nitrogen removal key enzymes of 27 Bacillus sp. isolates.
Fig 5.
+ -N, NO2--N, NO3--N, and TN by eight Bacillus sp. isolates in inorganic nitrogen degradation ability test medium. (B1, B6, B19, B20, B23, B24, B25 and B27) represent the denitrification performance of experimental strains B1, B6, B19, B20, B23, B24, B25 and B27, respectively. (CG) Changes in nitrogen content in the control group.
Fig 6.
Application effect of eight Bacillus sp. isolates on nitrogen removal in mariculture wastewater.
(B1, B6, B19, B20, B23, B24, B25 and B27) represent the nitrogen removal performance of experimental strains B1, B6, B19, B20, B23, B24, B25 and B27, respectively. (CG) variation of nitrogen content in the control group.