Table 1.
RNA-Seq libraries prepared from untreated (UT) and cloquintocet-mexyl (CM)-treated wheat leaf tissue.
Fig 1.
Mean-difference plot showing the log2 fold change and mean abundance of each transcript in log2 counts per million (CPM).
Genes that were significantly induced (Up) and repressed (Down) by cloquintocet-mexyl are highlighted in red and blue, respectively. Genes that were not significantly differentially expressed (Non-DE) are highlighted in black. TraesCS5A02G397800.1 is circled in green.
Fig 2.
Tree map of functional annotations assigned to significant differentially expressed genes identified by RNA-Seq.
The main categories (bolded and underlined) include Phase I (light green), Phase II (orange), and Phase III (grey) metabolism, Amino Acid Metabolism (light blue), Transcription Factors (TFs; yellow), Proteins with only Domain Info (dark blue), and Stress/Defense Related (dark green). The number of identified genes is listed in parentheses or after the comma. Abbreviations: 2OG, 2-oxoglutarate; 5β-PORs, progesterone 5-beta-reductase; ABC, ATP-binding cassette; ADH, alcohol dehydrogenase; AzoR, azoreductase; CCR, cinnamoyl-CoA reductase 4; CSE, cystathionine gamma-lyase; CYP, cytochrome P450; GST, glutathione S-transferase; GT, glycosyltransferase; HTH, helix-turn-helix; IP, inhibitor protein; LAC, laccase; MTOX, N-methyl-L-tryptophan oxidase; OPR, 12-oxophytodienoate reductase; PLATZ, plant AT-rich protein and zinc-binding protein; PRP, pathogen-related protein; SBP, selenium-binding protein; SCP, serine carboxypeptidase; SDR, short chain dehydrogenase/reductase.
Fig 3.
Mean fold changes for CYP81A-5A, CYP81A-5B, and CYP81A-5D in response to cloquintocet-mexyl (CM) and halauxifen-methyl (HM) at 3, 6, and 12 hours after treatment (HAT).
Treatments include untreated control (0.1% nonionic surfactant (NIS)), CM (15 g a.i. ha−1 of CM), HM (5 g a.e. ha−1 of HM) and CM+HM (15 g a.i. ha−1 of CM and 5 g a.e. ha−1 of HM). All treatments included 0.1% NIS. Within each timepoint values that share the same letter are not significantly different (α = 0.05). Fold inductions for each gene at each timepoint were calculated by 2(−ΔΔCt) with β-tubulin (β-TUB) as a reference gene. Mean fold changes represent results from three biological replicates (n = 3). Error bars represent standard error of the mean.