Fig 1.
Effect of follicular fluid (FF) supplementation during the maturation period on cumulus cell expansion.
(A) Mean area of cumulus oocyte complexes (COCs; n = 782; μm2) at 0, 6, 12 and 19 h of the maturation period. Cumulus oocyte complexes were matured in oocyte maturation medium supplemented with 0% (cOMM), 25% (FF25), 50% (FF50), 75% (FF75) or 100% (FF100) of pooled FF. (B) Increase (%) in the area of COCs (n = 782) after maturation in designated concentrations of FF for 19 h. Data are presented as least squares mean ± SEM of the percentage of area increase from 0 h to 19 h. Different letters above the bars indicate significant differences between treatments (P≤0.03).
Fig 2.
Percentage of increase in the area of cumulus oocyte complexes (COCs).
Cumulus oocyte complexes (n = 300) were matured in oocyte maturation medium supplemented with 0% (cOMM), 50% or 75% of either small (SFF) or large (LFF) FF for 22 h. Results shown are least-squares means ± SEM. * P<0.05; ** P<0.01.
Table 1.
Area of cumulus oocyte complexes (COCs; μm2) at 0, 6, 12 and 19 h of the maturation period.
Cumulus oocyte complexes were matured in media containing 0%, 25%, 50%, 75% or 100% pooled follicular fluid. Data presented as least squares mean ± SEM (x100000).
Fig 3.
Effect of follicular fluid (FF) supplementation and time of maturation on development of embryos to the blastocyst stage.
Cumulus oocyte complexes (COCs; n = 2159) were exposed to oocyte maturation medium supplemented with 0% or 75% FF during the 12 h, 19 h or 22 h maturation period. Data is presented as percent putative zygotes that cleaved (A), percent of putative embryos that became blastocysts (B) or percent of cleaved embryos that became blastocysts (C). P values for the main effects of maturation time (Mat, h), FF supplementation (%) and the interaction are shown within graphs. Different letters above the bars indicate significant difference between treatments (P ≤ 0.02).
Fig 4.
Effect of supplementation of oocyte maturation medium (OMM) with follicular fluid (FF) from small or large follicles on development of embryos to the blastocyst stage.
Cumulus oocyte complexes (COCs; n = 692) were exposed to OMM supplemented with 0% (cOMM), 50% or 75% FF from small (SFF) or large (LFF) follicles during the 22 h maturation period. Data is presented as percent putative zygotes that cleaved (A), percent of putative embryos that became blastocysts (B) or percent of cleaved embryos that became blastocysts (C). Different letters above the bars indicate significant difference between treatments (P ≤ 0.03).
Table 2.
Percent of fertilized cumulus oocyte complexes (COCs) that cleaved and became blastocysts.