Fig 1.
Setup on a greenhouse table for the four densities tested in the density trial, exemplary for replicate 1.
The black rectangle represents the table. The colored circles show the positions of the plants. Black circles show the center plants that were used for measurements.
Fig 2.
Experimental design for the trial to test the effects of the duration of the vegetative phase (V-trial).
Fig 3.
Exemplary plants at final harvest for the density trial (D-trial) trial of increasing duration of vegetative growth phase (V-trial).
Scale = 15 cm.
Table 1.
Estimated slopes of the linear regression of target traits as a function of plant density (D-Trial) and length of the vegetative phase (V-Trial).
Fig 4.
Scatter plots of the linear regression for the relationship between plant density and A) dry leaf matter, B) dry stem matter, C) dry yield, and D) CBD yield in dependence on the position at the upper and lower plant half. Point symbols indicate the mean values for the respective plant halves. Error bars indicate the estimated standard error of the mean (n = 3). Dashed lines show the fitted linear regression models for each plant half. p-values indicate wether the estimated slope term was significantly different from zero.
Fig 5.
Scatter plots of the linear regression for the relationship between duration of vegetative phase and A) dry leaf matter, B) dry stem matter, C) dry yield, and D) CBD yield in dependence on the position at upper and lower plant half. Point symbols indicate the mean values for the respective plant halves. Error bars indicate the estimated standard error of the mean (n = 3). Dashed lines show the fitted linear regression models for each plant half. p-values indicate wether the estimated slope term was significantly different from zero.
Fig 6.
Scatter plots of the linear regression for the relationship between the proportion of individual plant organs in the total biomass with A) plant density and B) duration of the vegetative phase. Point symbols indicate the mean values for the respective plant organs. Error bars indicate the estimated standard error of the mean (n = 3). Dashed lines show the fitted linear regression models for each plant organ based on the separate regression analyses. For A): p-values result from the global F-test for the fixed effect of plant density. For B): The p-values indicate whether the estimated slope terms of the final harvest are significantly different from zero.
Table 2.
Estimated intercepts of the regression analysis for CBD concentration and average inflorescence mass in dependence on inflorescence fractions (intercept) in the D-trial.
Fig 7.
Scatter plots of the linear regression for the relationship between the duration of the vegetative phase and the average mass of single inflorescences.
Point symbols indicate the mean values for the respective plant organs. Error bars indicate the estimated standard error of the mean (n = 3). Dashed lines show the fitted linear regression models for the inflorescence fractions of the main apical inflorescence (MAI) and axillary inflorescences of the upper and lower plant half. The p-values indicate whether the estimated slope terms of the final harvest are significantly different from zero.
Table 3.
Mean CBD concentration (%) for duration of vegetative phase (DVP) and inflorescence fraction.
Fig 8.
Scatter plots of the relationship between plant density and A) maximum photosynthetic rate (Amax), B) specific leaf area (SLA), C) red/far-red ratio, and D) fraction intercepted light. Point symbols show the estimated means for the respective plants, grown under densities of 12, 16, 24, and 36 plants m-2. Error bars indicate the estimated standard error of the mean (n = 3). For A), B), C): Dashed lines show the fitted linear regression models in dependence on A) the uppermost fully developed leaf (upper) and uppermost leaf of the lower plant half (lower), B) leaves of upper and lower plant half, and C) measurements at medium canopy height (middle) and pot height (bottom). The p-values indicate whether the estimated slope terms are significantly different from zero. For D): Dashed lines show the fitted logistic functions for the tested plant densities.
Table 4.
Estimated mean values for light interception.
Fig 9.
Scatter plots of the linear regression for the relationship between duration of the vegetative growth phase and A) total plant biomass per square meter at final harvest B) leaf area index (LAI) at third harvest, C) dry yield per square meter at final harvest, and D) CBD yield per square meter at final harvest. Point symbols show the estimated means for the respective plants, grown in the vegetative phase for 1,2,3, and 4 weeks. Error bars indicate the estimated standard error of the mean (n = 3). The solid line shows the fitted linear regression model. The p-values indicate whether the estimated slope terms of the final harvest are significantly different from zero.
Fig 10.
Scatter plots of the linear regression for the relationship between plant density and A) total plant biomass per square meter B) leaf area index (LAI), C) dry yield per square meter, and D) CBD yield per square meter. Point symbols show the estimated means for the respective plants, grown under densities of 12, 16, 24, and 36 plants m-2. Error bars indicate the estimated standard error of the mean (n = 3). The solid line shows the fitted linear regression model. p-values result from the global F-test for the fixed effect of plant density.