Table 1.
Composition of different proniosomal formulations.
Table 2.
Primer sequences of target genes.
Table 3.
Physiochemical characterization of formulations (Mean ± SD).
Fig 1.
Optical photography (A), Transmission electron microscopy (B), and release profile of the chosen formula (C).
Fig 2.
(A) Gross examination of wound area of the experimental groups, and (B) Wound diameter during the experiment period for the experimental groups.
Data are presented as mean ± SD.
Table 4.
Changes in total antioxidant capacity (TAC) and malondialdehyde (MDA) in the tested groups.
Fig 3.
Changes in mRNA expression of iNOS (A) TNF-α (B) IL-1β (C), and MMP-9 (D) genes in the different groups. Data are expressed as mean ± SE (n = 4).
Means with distinct letters in the same column are significantly dissimilar at p < 0.05.
Fig 4.
Changes in expression of macrophage activation-related genes, CD68 (A) and CD163 (B) in the experimental groups.
Data are expressed as mean ± SE (n = 4). Means with distinct letters in the same column are significantly dissimilar at p<0.05.
Fig 5.
Photomicrograph of skin wound (H&E-stained sections), (a) control group on day 7 showed intense inflammatory cell infiltration and necrosis, (b) TAD group on day 7 showed intense inflammatory cell infiltration with necrosis, (c) DEX group, on day 7 showed intense inflammatory cell infiltration, edema, and scab formation, (d) TAD+DEX group at day 7 showing scab covered wound with intense inflammatory cell infiltration, (e) The control group on day 14 showed scab-covered wounds consisting of necrotic tissue and inflammatory cells, underlying tissue showing inflammatory cell infiltration, and unorganized granulation tissue, (f) TAD group on day 14 showed fewer inflammatory cell infiltration with well-arranged organized tissue, (g) DEX group, on day 14 showed intense inflammatory cell infiltration with necrosis, (h) TAD+DEX group on day 14 showed few inflammatory cells and organized tissue formation, (i) The control group on day 21 showed moderate inflammatory cell infiltration, and unorganized granulation tissue with no evidence of re-epithelialization, (j) The TAD group on day 21 showed re-epithelialization with scarce inflammatory cell infiltration and well-formed granulation tissue, (k) DEX group, on day 21 showed moderate inflammatory cell infiltration with unorganized granulation tissue, and (l) TAD+DEX group at day 21 showing mild inflammatory cell infiltration, and re-epithelialization with granulation tissue formation. (scale bar 100 μm).
Table 5.
Wound healing criteria of the experimental groups.
Fig 6.
Photomicrograph of skin wound (MTC stained sections), (a) control group at day 7 showed few collagen fibers in the wound area. (b) The TAD group on day 7 showed a moderate amount of collagen. (c) DEX group, on day 7 showed few collagen fibers. (d) the TAD+DEX group on day 7 showed few collagen fibers formation. (e) The control group on day 14 showed few collagen fibers in the wound area. (f) The TAD group on day 14 showed an increase in the amount of collagen fiber. (g) DEX group, on day 14 showed disorganized collagen fibers. (h) TAD+DEX group on day 14 showed increased collagen formation. (i) The control group on day 21 showed moderate disoriented collagen fiber. (j) The TAD group on day 21 showed a high amount of well-organized collagen fiber. (k) DEX group, on day 21 showing disorganized collagen fibers. (l) The TAD+DEX group on day 21 showed moderate collagen fiber amount. (scale bar 100 μm). (m) area % of collagen fiber in different treated groups (data was expressed as mean ± SE, different letters indicating significant differences at p < 0.05). TAD; Tadalafil-Loaded Proniosomal Gel, DEX; Dexamethasone.
Fig 7.
Photomicrograph of skin wound (TGF-β1 stained sections), (a) control group at day 7 showing weak immune expression. (b) The TAD group on day 7 showed moderate immune expression. (c) DEX group, on day 7 showed weak immune expression. (d) TAD+DEX group on day 7 showed weak immune expression. (e) The control group on day 14 showed moderate immune expression. (f) The TAD group on day 14 showed moderate immune expression. (g) DEX group, on day 14 showed moderate immune expression. (h) TAD+DEX group on day 14 showed moderate immune expression. (i) Control group at day 21 showing moderate immune expression (j) TAD group at day 21 showing strong immune expression. (k) DEX group, on day 21 showed moderate immune expression. (l) the TAD+DEX group on day 21 showed strong immune expression. (scale bar 100 μm). (m) area % of TGF-β1 expression in different treated groups (data was expressed as mean ± SE, different letters indicating significant differences at p < 0.05). TAD; Tadalafil-Loaded Proniosomal Gel, DEX; Dexamethasone.
Fig 8.
Photomicrograph of skin wound (VEGF-stained sections).
(a) the control group on day 7 showed weak immune expression. (b) The TAD group at day 7 showed moderate immune expression. (c) DEX group, on day 7 showed weak immune expression. (d) TAD+DEX group on day 7 showed weak immune expression. (e) The control group on day 14 showed moderate immune expression. (f) The TAD group on day 14 showed moderate immune expression. (g) DEX group, on day 14 showed moderate immune expression. (h) TAD+DEX group on day 14 showed moderate immune expression. (i) the control group on day 21 showed moderate immune expression (j) the TAD group on day 21 showed strong immune expression. (k) DEX group, on day 21 showing moderate immune expression. (l) TAD+DEX group on day 21 showing strong immune expression. (scale bar 100 μm). (m) area % of VEGF expression in different treated groups (data was expressed as mean ± SE, different letters indicating significant differences at p < 0.05). TAD; Tadalafil-Loaded Proniosomal Gel, DEX; Dexamethasone.