Fig 1.
Photographs showing representative immunohistochemical staining patterns of CD4, CD8, CD68, and L1/MAC387 in HGSOC cells.
Original magnification: High magnification, × 400. Scale bar: 25 μm, magnification, × 400.
Fig 2.
Representative immunohistochemical staining patterns of PD-L1 and PD-L2 in HGSOC cells and distribution regarding patient’s platinum resistance, overall survival, and primary debulking surgery status.
In Fig 2a, the expression of PD-L1 and PD-L2 was categorized as: (A) 0 - negative expression; (B) 1+ weakly positive expression; (C) 2+ moderately positive expression; and (D) 3+ stronger positive expression. Original magnification: A-H, x 400; Scale bar: 25μm, magnification. In Fig 2b, the expression profile of PD-L1 and PD-L2 is presented regarding the patient’s platinum resistance, overall survival and primary debulking surgery status.
Fig 3.
Distribution of the somatic variants in HR-Related genes considering Platinum response.
In this chart, we compare the distribution of somatic variants found in genes involved in HR DNA Repair between PS (left) and PR (right) groups. Each gene is represented by a unique color. Variants are divided by genes in the inner circle and subdivided by ClinVar classification in the outer.
Fig 4.
Platinum-response classifier built with annotated variants and immunological markers.
(a) Supervised decision tree classifier analysis selected two variables as important for patients classification regarding PtC-response: Most PS patients were characterized by absence of the CHEK2-DIV (n = 8), while PR patients were characterized by CHEK2-DIV presence and higher levels of L1 (n = 5). (b) Coherently, CHEK2-DIV absence was significantly associated with Pt-C sensitivity (p < 0.05) and (c) L1 expression was significantly lower in PS patients with CHEK2-DIV.
Fig 5.
(a) The CoxPH regression analysis with the markers analyzed in this study. (b-d) Adjusted survival curves for the CoxPH Model for selected variables illustrate the differences in patients’ 5-year OS regarding Pt-C resistance, PD-L2 expression and CHEK2-DIV presence. Significance analyses were performed using the Wald test.