Fig 1.
Morphological characteristics of bulbils at different growth stages.
Fig 2.
Determination of carbohydrate content and related enzyme activities in Pinellia ternata bulbils at different growth stages.
A. Determination of sucrose content at different stages. B. Determination of starch content at different stages. C. Determination of SuSy activity at different stages. D. Determination of SPS activity at different stages. E. Determination of SS activity at different stages. The x-axis displays different growth stages, while the y-axis represents the content or activity of the measured substance.
Fig 3.
Determination of endogenous hormone content in Pinellia ternata bulbils at different growth stages.
A. Determination of GA content at different stages. B. Determination of ABA content at different stages. C. Determination of JA content at different stages. D. Determination of IAA content at different stages. The x-axis represents different growth stages, while the y-axis represents the content of the measured substance.
Fig 4.
Statistics of transcriptome sequencing results of Pinellia ternata bulbils at different growth stages on the Illumina platform.
A. The number of annotations of the spliced unigene sequences in the NR, Swiss-Prot, KEGG, KOG, eggNOG, GO, and Pfam databases. B. The number of DEGs in different comparison groups. C. Venn diagram of a single gene in each group. D. Species distribution of the identified transcripts in the NR database. E. Correlation analysis of the qRT-PCR and RNA sequencing (RNA-Seq) results for 9 genes.
Fig 5.
KEGG enrichment analysis of gene expression pathways in Pinellia ternata bulbils at different growth stages.
Fig 6.
Heat maps of DEGs related to sucrose and starch synthesis in the bulbils at different growth stages plotted by log2 transformation of FPKM values.
Fig 7.
Heat maps of DEGs related to endogenous hormone metabolism in the bulbils at different growth stages plotted by log2 transformation of FPKM values.
A. ABA. B. GA. C.IAA. D.JA.
Fig 8.
DEGs related to the TF family in the bulbils at different growth stages.
A. The number of DEGs at different growth stages. B. Heat maps of DEGs at each stage.
Fig 9.
DEGs related to plant hormone signal transduction in the bulbils at different growth stages.
A. Plant hormone signal transduction pathway B. Heat maps of DEGs at different growth stages.
Fig 10.
Gene co-expression network analysis of 15 samples of bulbils at different growth stages.
A. TOM correlation heat maps for all transcripts. B. Co-expression network modules constructed by transcripts. Different colors represent the network modules constructed, and the phylogenetic tree represents the hierarchical clustering of different samples, where each color corresponds to one module. C. Transcript expression profiles in the co-expressed blue2 module. D. Transcript expression profiles in the co-expressed dark olive green module. In the histogram, the x-axis represents all samples, and the y-axis represents the expression profile of the transcripts in the module.
Fig 11.
Core transcripts that were significantly associated with changes in endogenous metabolite content of sucrose, starch, SPS, SuSy, SS, IAA, JA, GA and ABA were screened in the co-expression module.
A. Correlation heat maps of the identified co-expression module with the content of endogenous metabolites in the bulbils. B. Gene co-expression network of the identified antiquewhite2 module related to the content of IAA and ABA. C. Gene co-expression network of the identified blue2 module related to the content JA and ABA. D. Gene co-expression network of the identified dark olive green module related to the content of starch. E. Gene co-expression network of the identified honeydew module related to the content of sucrose, JA, ABA and SPS, SuSy, SS.