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Table 1.

Augmented ANOVA for 10 quantitative traits of adzuki bean for season 1 (2021) and season 2 (2022).

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Table 2.

Descriptive statistics of quantitative traits of adzuki bean for both season (2020 & 2021).

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Table 3.

Genetic variability analysis for 10 quantitative traits of adzuki bean for both the season 1 (2020) and 2 (2021).

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Table 4.

List of promising accessions of adzuki bean with desirable traits based on morphological characterization.

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Table 5.

Contributions of variables for each principal component of PCA.

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Fig 1.

PCA biplot indicating the distribution of 10 quantitative traits in 100 accessions of adzuki bean based on their calculated component loading values.

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Fig 2.

Correlation matrix of quantitative traits where; DFF: Days to 50% flowering, PB: Primary branches, CLP: Number of clusters per plant, PPC: Number of pods per cluster, PPP: Number of pods per plant, PH: Plant height, DM: Days to 80% of maturity, SYPP: Seed yield per plant, SW: Seed weight, SPP: Number of seeds per pod. Ns = non-significant, *p < 0.05, **p < 0.01, ***p< 0.001.

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Fig 3.

“k- means clustering” of 100 accessions and three checks of adzuki bean based on ten quantitative traits.

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Table 6.

The number of accessions and average values of five clusters for ten different quantitative traits for 100 accessions of adzuki bean.

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Fig 4.

A&B: Representing variations in plant growth habits observed in adzuki bean accessions A- erect growth habit (EC15256); B- semi-spreading growth habit (EC34264).

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Fig 5.

Representing variations in stem colour of adzuki bean.

A- purple coloured stem (EC340271); B- Green coloured stem (IC360528).

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Fig 6.

Figure representing diversity in seed coat colour in adzuki bean molecular characterization using SSR.

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Table 7.

List of SSR primers used for genotyping of adzuki bean and their allelic diversity.

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Fig 7.

Dendrogram based on Nei genetic diversity using neighbor joining tree method of clustering, depicting genetic diversity and relationship between 100 accessions of adzuki bean as revealed by polymorphism of 22 SSRs.

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Fig 8.

Model-based clustering of 100 accessions of adzuki bean detected based on 22 SSR markers.

An accession is represented by each bar. The various colour bars (red, green and blue respectively) reflect various genetic groups, while the combination of colours in a bar indicates admixture.

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Table 8.

Analysis of molecular variance (AMOVA) of 100 accessions of adzuki bean.

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Fig 9.

Principal Coordinate Analysis (PCoA) of 100 accessions of Vigna angularis.

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