Fig 1.
CY and DEX significantly inhibited the immune function of DNFB induced sensitized mice.
(A-B) Thymus index and spleen index of mice in Contorl, CY and DEX groups. (C) Structural diagram of thymus and MLNs of mice in Contorl, CY and DEX groups. (D) Intestinal sIgA content of mice in Contorl group, CY group and DEX group. (E) Degree of ear swelling in mice in Contorl group, CY group and DEX group. (F) HE staining was used to detect the degree of infiltration of ear inflammatory cells in mice. Black arrows indicate infiltrating inflammatory cells. Scale: 50 μm. (G) The number of intestinal PPs in mice in Contorl, CY and DEX groups. (H) HE staining was used to detect the degree of infiltration of intestinal PPs inflammatory cells in mice. Scale: 100 μm. Compared to the Control group, **P<0.01, ***P<0.001. Compared to the DEX group, ##P<0.01.
Fig 2.
CY and DEX significantly reduced lymphocyte proliferation in Con A induced sensitized mouse MLNs.
MTT was used to detect lymphocyte proliferation in MLNs sensitized mice in the Contorl, CY, and DEX groups. Compared to the Control group, ***P<0.001. Compared to the DEX group, ##P<0.01.
Fig 3.
Percentage of CD3+ total T cells and CD3+CD69+ activated T cells in mesenteric lymph nodes (MLNs) of sensitized mice were detected by flow cytometry.
Representative flow cytomogram of CD3+ total T cells and CD3+CD69+ activated T cells in mouse MLNs in Contorl, CY and DEX groups and corresponding statistical analysis. Compared to the Control group, **P<0.01, ***P<0.001. Compared to the DEX group, #P<0.05, ###P<0.001.
Fig 4.
Flow cytometry was used to detect the distribution of CD3+CD4+ helper T cells in the mesenteric lymph nodes (MLNs) of sensitized mice.
Representative flow cytometry of CD3+CD4+ helper T cells in mouse MLNs in Contorl, CY and DEX groups and corresponding statistical analysis. Compared to the Control group, **P<0.01. Compared to the DEX group, ##P<0.01.
Fig 5.
Flow cytometry was used to detect the distribution of CD3+CD8+ cytotoxic T cells in the mesenteric lymph nodes (MLNs) of sensitized mice.
Representative flow cytomogram of CD3+CD8+ cytotoxic T cells in mouse MLNs in Contorl, CY and DEX groups and corresponding statistical analysis.
Fig 6.
Flow cytometry was used to detect the distribution of CD3+TCRβ+ T cells in the mesenteric lymph nodes (MLNs) of sensitized mice.
Representative flow cytometry of CD3+TCRβ+ T cells in mouse MLNs in Contorl, CY and DEX groups and corresponding statistical analysis. Compared to the Control group, **P<0.01. Compared to the DEX group, ##P<0.01.
Fig 7.
Flow cytometry was used to detect the distribution of CD3+TCRγ/δ+ T cells in the mesenteric lymph nodes (MLNs) of sensitized mice.
Representative flow cytometry of CD3+TCRγ/δ+ T cells in mouse MLNs in Contorl, CY and DEX groups and corresponding statistical analysis. Compared to the Control group, *P<0.05, ***P<0.001. Compared to the DEX group, ###P<0.001.
Fig 8.
Analysis results of intestinal flora diversity in mice.
(A) Venn diagram was used to show the common and unique operational taxa (OTU) of intestinal samples from different treatment groups. (B) and (C) showed the Shannon and Chao indices of intestinal microflora in control group, CY group and DEX group, respectively, to assess α diversity. *P<0.05, **P<0.01, ***P<0.001.
Fig 9.
Analysis of intestinal flora structure in mice.
(A) Relative abundance of phylum-grade dominant bacteria in fecal samples of mice in control group, CY treatment group and DEX treatment group. From bottom to top, in order, are: P_Bacteroidota, p_Firmicutes, p_Campylobacterota, p_Deferribacterota, P_Patescibacteria, p_Proteobacteria, p_Desulfobacterota and other. (B) Analysis of intestinal flora composition at genus level in fecal samples of mice in control group, CY treatment group and DEX treatment group. The top ten, are as follows:g_Bacteroides, g_norank_Muribaculaceae, g_Lachnospiraceae_NK4A136_group, g_Helicobacter, g_Alloprevotella, g_Alistipes, g_unclassified_Lachnospiraceae, g_norank_Oscillospiraceae, g_Parabacteroides and g_norank_Clostridia_UCG-014.