Table 1.
Mycorrhization parameters (mean data) of AMF starters used for inoculum production on the pilot site.
Fig 1.
Plan and stapes of scaling-up of AMF inoculum production on pilot site.
a, not crushed peanut shells; b, shell grinding mill; c, sterilized material (metal barrels); d, plastic containers placed in greenhouse; e, sterile crushed peanut shells into plastic containers; f, soaked corn seeds; g, growing corn plants; h, collected multiplied inoculum (substrate and roots).
Table 2.
Characteristics of the inoculum produced on agricultural residues alone or mixed with sand in Leonard jars.
Table 3.
Characteristics of the inoculum produced on agricultural residues in pots.
Table 4.
Characteristics of the inoculum produced on the peanut shell at the pilot site.
Table 5.
Chemical composition of peanut shell before and after the inoculum production.
Fig 2.
Box plot showing the effect of using salt water and substrate sterilization on inoculum quality parameters (Spore number, mycorrhization intensity and frequency).
LCM refers to inoculation with the mix of LCM strains; T, uninoculated; Salty, irrigated with salted water (NaCl, 0.5% W/V); No Salty, irrigated with unsalted water; Sterile, sterilized peanut shell support; Non_Sterile, unsterilized peanut shell support.
Fig 3.
Principal component analysis showing the effects of the treatments on the mycorhization using as variables the number of spores, mycorrhization intensity and frequency.
(:) means combination between treatments; LCM:No Salty:No Sterile, unsterilized support inoculated and watered with unsalted water; LCM:Salty:No Sterile, unsterilized support, inoculated and watered with salt water; LCM:No Salty:Sterile, sterilized support, inoculated and watered with unsalted water; LCM:SaltySterile, sterilized support, inoculated and watered with salt water; T:Salty:Sterile, sterilized support, not inoculated and watered with salt water; T:No_Salty:No Sterile, unsterilized support, uninoculated and watered with unsalted water; T:No_Salty:Sterile, sterilized support, not inoculated and watered with non-salty water and T:Salty:No Sterile, unsterilized support, uninoculated and watered with salt water.
Fig 4.
Mean number of spores obtained by classical and improved extraction methods when using peanut shells as growth substrate.
The means of three repetitions per treatment followed by different letters differ according to the Fisher test (p = 0.05). Bars represent standard deviations from the mean (n = 3). The comparison is made within each treatment. INO, inoculum originated from the Darou Mousty pilot site obtained with salted and unsalted irrigation water; LCM, inoculum obtained from starter of Joint Microbiology Laboratory (LCM) on peanut shells in the laboratory; control, uninoculated corn grown in peanut shell.